Teleost GSDMEc regulates GSDMEa-mediated pyroptosis
文献类型：期刊论文
| 作者 | Xu, Hang2,3; Wu, Meng1,2,3; Wang, Yujian2,3; Jiao, Yaoming1,2,3; Chen, Yuan1,2,3; Yuan, Zihao1,2,3; Sun, Li1,2,3 |
| 刊名 | JOURNAL OF ADVANCED RESEARCH
 |
| 出版日期 | 2026 |
| 卷号 | 79页码:105-119 |
| 关键词 | Gasdermin Caspase Pyroptosis Teleost |
| ISSN号 | 2090-1232 |
| DOI | 10.1016/j.jare.2025.04.007 |
| 通讯作者 | Yuan, Zihao(yuanzihao@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Introduction: Gasdermin (GSDM) is a family of proteins that execute pyroptosis after being activated by caspase (CASP) cleavage. Mammals possess five GSDM members (A-E) with pyroptotic ability. Teleosts possess only one pyroptotic GSDM, GSDME, that exists in three orthologs, GSDMEa, b, and c. GSDMEa and GSDMEb are known to induce pyroptosis, but the function of GSDMEc is unknown. Objectives: The present study aimed to elucidate the function of teleost GSDMEc and examine the interplay among teleost GSDME orthologs by using snakehead Channa argus as a representative species. Methods: Pyroptosis was assessed via microscopy and biochemical assays. GSDME cleavage, oligomerization, and membrane translocation were examined via immunoblotting. The interactions of GSDME products were examined using confocal microscopy and co-immunoprecipitation. GSDME knockdown in fish and in vivo bacterial infecti Results: C. argus possessed was cleaved by C. argus CASP (CaCASP) 1/8 to produce an N-terminal fragment (NT), NT261, that induced pyroptosis. CaGSDMEc1 an and NT108, respectively, were unable to induce pyroptosis. However, both NT123 and NT108 could bind and promote the pyroptot translocation. The interacti that is conserved in the me pyroptotic activity of N CaGSDMEc1/c2 cleavage in snakehead cells, resulting in pyroptosis, IL-1b/18 maturation cleavage, and on were performed. three GSDME variants (CaGSDMEa, CaGSDMEc1,and CaGSDMEc2). CaGSDMEa d CaGSDMEc2 were also cleaved by CaCASP1/8, but the resulting NTs, NT123 ic activity of NT261 by facilitating NT261 oligomerization and membrane on between NT261 and NT123/NT108 depended on a positively charged motif tazoan GSDME and is essential to the membrane localization of NT123 and the T261. Bacterial infection induced CaGSDMEa/CaCASP8 activation and extracellular DNA-net for tion in fish tissues and red mation. CaGSDMEa/c1 knockdown significantly increased bacterial disseminad fish survival. led the functions and interactive mechanism of teleost GSDME orthologs, uce Conclusions: Our results revea and provided new insights into the regulation of pyroptosis in lower vertebrates. (c) 2025 The Author(s). Pub lished by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| WOS关键词 | GASDERMIN D ; MECHANISM ; INFLAMMASOME ; CLEAVAGE ; CASPASES ; BINDING |
| 资助项目 | Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203000] ; China Postdoctoral Science Foundation[2023TQ0356] ; Postdoctoral Fellowship Program of China Postdoctoral Science Foundation[GZC20232709] ; National Natural Science Foundation of China[41576150] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001645004000001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204499]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yuan, Zihao; Sun, Li |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Hang,Wu, Meng,Wang, Yujian,et al. Teleost GSDMEc regulates GSDMEa-mediated pyroptosis[J]. JOURNAL OF ADVANCED RESEARCH,2026,79:105-119. |
| APA | Xu, Hang.,Wu, Meng.,Wang, Yujian.,Jiao, Yaoming.,Chen, Yuan.,...&Sun, Li.(2026).Teleost GSDMEc regulates GSDMEa-mediated pyroptosis.JOURNAL OF ADVANCED RESEARCH,79,105-119. |
| MLA | Xu, Hang,et al."Teleost GSDMEc regulates GSDMEa-mediated pyroptosis".JOURNAL OF ADVANCED RESEARCH 79(2026):105-119. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。