Biochemical characterization of mannuronan C5-epimerase from Saccharina japonica
文献类型：期刊论文
| 作者 | Meng, Xue1,2,3; Li, Shuang1,2,3; Zhang, Jie2,3; Duan, Delin2,3 |
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2026-01-14 |
| 页码 | 12 |
| 关键词 | alginate
mannuronan C5-epimerase
|
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-025-5173-2 |
| 通讯作者 | Duan, Delin(dlduan@qdio.ac.cn) |
| 英文摘要 | Alginate is a polysaccharide predominantly synthesized by brown algae and bacteria. It is a linear polymer composed of beta-D-mannuronic acid (M) and its C5 reverse isomer alpha-L-guluronic acid (G). Currently, there is a significant demand for high-G-content alginate in the market. Mannuronan C5-epimerase (MC5E) is one important enzyme in the alginate synthesis as it catalyzes epimerization of beta-D-mannuronic acid (M) to alpha-L-guluronic acid (G) at the polyM polymer level. We screened and isolated one high expression MC5E sequence from the Saccharina japonica genome database and demonstrated that SjMC5E1 could epimerize and increase the content of G residues in alginate. Additionally, we detected the mannuronan C5-epimerase activity of SjMC5E1 was optimal at 37 degrees C in the temperature range of 15-50 degrees C, the FG was 0.30 and the M/G ratio of alginate was the lowest at 2.32. The biochemical characterization of SjMC5E1 provides valuable insights into its role in alginate biosynthesis, particularly in the conversion of M to G. These findings contribute to a better understanding of the enzymatic mechanisms underlying alginate synthesis and may support future efforts to optimize alginate production. However, further studies are needed to fully explore the potential of SjMC5E1 in tailoring alginates with specific properties for industrial or biomedical applications. |
| WOS关键词 | BROWN ALGA ECTOCARPUS ; ALGINATE BIOSYNTHESIS ; SEQUENCE ; C-5-EPIMERASE ; MODEL ; PHAEOPHYCEAE ; SPECTROSCOPY ; EXPRESSION ; TOPOLOGY ; ACID |
| 资助项目 | International Partnership Program of Chinese Academy of Sciences[133137KYSB20210034] ; Qingdao International Collaboration Project[22-3-6-ghgg-1-hz] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001661388100001 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204577]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Duan, Delin |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Key Lab Breeding Biotechnol & Sustainable Aquacult, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Meng, Xue,Li, Shuang,Zhang, Jie,et al. Biochemical characterization of mannuronan C5-epimerase from Saccharina japonica[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2026:12. |
| APA | Meng, Xue,Li, Shuang,Zhang, Jie,&Duan, Delin.(2026).Biochemical characterization of mannuronan C5-epimerase from Saccharina japonica.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,12. |
| MLA | Meng, Xue,et al."Biochemical characterization of mannuronan C5-epimerase from Saccharina japonica".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2026):12. |
入库方式： OAI收割
来源：海洋研究所
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