Ninjurin-1 executes plasma membrane rupture and impairs anti-microbial immunity in an early jawed vertebrate
文献类型：期刊论文
| 作者 | Jin, Xiaotong2,3,4; Yuan, Liming1,2; Sun, Chunyan1,2; Jiang, Shuai2,3,4 |
| 刊名 | CELLULAR AND MOLECULAR LIFE SCIENCES
 |
| 出版日期 | 2025-12-23 |
| 卷号 | 83期号:1页码:18 |
| 关键词 | Teleost Ninjurin Plasma membrane rupture Cell death Immunity. |
| ISSN号 | 1420-682X |
| DOI | 10.1007/s00018-025-06020-0 |
| 通讯作者 | Jiang, Shuai(sjiang@qdio.ac.cn) |
| 英文摘要 | Plasma membrane rupture (PMR), once considered a passive event in necrosis, is actively executed by ninjurin-1 (NINJ1). Although PMR is a well-documented phenomenon across animal species, its molecular mechanism and immunological function in early diverging jawed vertebrates remain unclear. Herein, we identify a functional homolog of NINJ1 in turbot. Ectopic expression of turbot NINJ1 effectively lyses the plasma membrane, leading to rapid PMR and massive release of cellular contents. Given that turbot NINJ1 has two transmembrane helices, it could distribute within membrane-bound organelles and the plasma membrane as PMR progresses. Co-expression with turbot gasdermin significantly amplifies NINJ1-mediated PMR, even when both proteins are present at non-cytotoxic levels. Structural analysis reveals the importance of its extracellular and transmembrane helices, with specific functional residues crucial for its PMR-inducing activity. Bacterial infection upregulates NINJ1 and activates pyroptosis signaling pathway, and provokes strong proinflammatory responses. Inhibiting NINJ1 improves fish survival, whereas blocking pyroptosis actually dampens host antimicrobial immunity. Collectively, the present findings delineate a mechanistically distinct role of NINJ1 in regulating programmed cell death in turbot, highlighting the complex and bifunctional nature of cell death in teleost immunity. |
| WOS关键词 | PROGRAMMED CELL-DEATH ; GASDERMIN D ; PYROPTOSIS ; INFLAMMASOME ; APOPTOSIS ; INJURY ; PANOPTOSIS ; RELEASE ; KIDNEY |
| 资助项目 | National Natural Science Foundation of China[32322085] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001662864900002 |
| 出版者 | SPRINGER BASEL AG |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204583]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Jiang, Shuai |
| 作者单位 | 1.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Univ Chinese Acad Sci, Sch Marine Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jin, Xiaotong,Yuan, Liming,Sun, Chunyan,et al. Ninjurin-1 executes plasma membrane rupture and impairs anti-microbial immunity in an early jawed vertebrate[J]. CELLULAR AND MOLECULAR LIFE SCIENCES,2025,83(1):18. |
| APA | Jin, Xiaotong,Yuan, Liming,Sun, Chunyan,&Jiang, Shuai.(2025).Ninjurin-1 executes plasma membrane rupture and impairs anti-microbial immunity in an early jawed vertebrate.CELLULAR AND MOLECULAR LIFE SCIENCES,83(1),18. |
| MLA | Jin, Xiaotong,et al."Ninjurin-1 executes plasma membrane rupture and impairs anti-microbial immunity in an early jawed vertebrate".CELLULAR AND MOLECULAR LIFE SCIENCES 83.1(2025):18. |
入库方式： OAI收割
来源：海洋研究所
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