Knockout of pilA gene in Spirulina platensis enhances the exopolysaccharide production and auto-aggregation
文献类型：期刊论文
| 作者 | Han, Yuxi1,2,3,4; Xie, Xiujun1,2,3; Wu, Songcui1,2,3; Wang, Shurui1,2,3; Gao, Shan1,2,3; Gu, Wenhui1,2,3; Wang, Guangce1,2,3 |
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2026-02-03 |
| 页码 | 13 |
| 关键词 | |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-025-5336-1 |
| 通讯作者 | Wang, Guangce(gcwang@qdio.ac.cn) |
| 英文摘要 | Spirulina, a commercially significant cyanobacterium, also named as Arthrospira or Limnospira, is widely used in food, feed, and pharmaceutical industries due to its rich protein content, phycocyanin, and exopolysaccharides (EPS). The role of the pilA gene was studied to encode the major pilin subunit of the type IV pilus (T4P) system in EPS synthesis and cell aggregation using the industrial strain Spirulina platensis FACHB-439. Through targeted knockout of pilA, we demonstrated that mutants exhibited enhanced EPS production and improved auto-aggregation under static conditions, despite losing phototaxis properties. Transcriptomic analysis revealed the coordinated up-regulation of genes involved in synthesis of partial T4P components (PilB, HpsC, etc.) and EPS (e.g., glycosyltransferase), and nitrogen recycling (Urease E/F), indicating adaptive metabolic responses to T4P deficiency. These findings showed the presence of integrating regulators linking the T4P system with EPS biosynthesis, nitrogen metabolism, and redox homeostasis. This study demonstrated that genetic knockout of pilA can be used to enhance EPS production and aggregation, offering a proof-of-concept for trait improvement in S. platensis, and providing a theoretical foundation and technical roadmap for the future development of industrial algal strains with superior performance. |
| WOS关键词 | GLIDING MOTILITY ; BIOGENESIS ; SUPPLEMENT ; SECRETION ; GROWTH |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDB1290000] ; National Key R&D Program of China[2018YFD0901500] ; National Key R&D Program of China[2024YFD2402003] ; Research Fund for the Taishan Scholar Project of Shandong Province[tspd20210316] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001677147000001 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204652]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guangce |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266000, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Han, Yuxi,Xie, Xiujun,Wu, Songcui,et al. Knockout of pilA gene in Spirulina platensis enhances the exopolysaccharide production and auto-aggregation[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2026:13. |
| APA | Han, Yuxi.,Xie, Xiujun.,Wu, Songcui.,Wang, Shurui.,Gao, Shan.,...&Wang, Guangce.(2026).Knockout of pilA gene in Spirulina platensis enhances the exopolysaccharide production and auto-aggregation.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,13. |
| MLA | Han, Yuxi,et al."Knockout of pilA gene in Spirulina platensis enhances the exopolysaccharide production and auto-aggregation".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2026):13. |
入库方式： OAI收割
来源：海洋研究所
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