Caspase-mediated interleukin-18 maturation promotes membrane-associated unconventional secretion and signal transduction in a teleost fish
文献类型：期刊论文
| 作者 | Yuan, Liming1,2; Xu, Hang1,3,4; Jiang, Shuai1,3,4 |
| 刊名 | MARINE LIFE SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2026-02-03 |
| 页码 | 14 |
| 关键词 | Teleost fish Interleukin-18 Unconventional secretion Innate immunity |
| ISSN号 | 2096-6490 |
| DOI | 10.1007/s42995-025-00335-4 |
| 通讯作者 | Jiang, Shuai(sjiang@qdio.ac.cn) |
| 英文摘要 | Interleukin-18 (IL-18) is an important proinflammatory cytokine essential for immune modulation. Unlike most cytokines, it is synthesized as an inactive precursor, with its maturation and secretion being critical for its functionality. As an evolutionarily ancient cytokine, it can be traced back to teleosts, but not zebrafish. However, the regulatory mechanism of IL-18 in early vertebrates remains largely elusive. The present study reports the maturation and secretion of IL-18 along with its role in signal transduction in a teleost fish half-smooth tongue sole (Cynoglossus semilaevis). We found that pro-IL-18 was cleaved by caspase-1, caspase-3/7, and caspase-6 at different N-terminal sites, generating three forms of the mature IL-18. In contrast to the negatively charged pro-IL-18, the positively charged mature IL-18 is highly enriched in the cytoplasmic membrane. It is enclosed within membrane-associated microvesicles, which facilitate secretion to the extracellular milieu. Once secreted, it binds specifically to the IL-18 receptor alpha (IL-18R alpha) present on the cell surface and recruits IL-18R beta to form a functionally active heterotrimeric complex. Bacterial challenge induces the maturation and secretion of IL-18, which upregulates the expression of the proinflammatory cytokines. Activation of the IL-18-mediated signaling pathway enhances antimicrobial immunity and reduces infection-induced mortality. Our findings collectively reveal a unique mechanism of IL-18 maturation, unconventional secretion, and immune regulation in a teleost fish, which provides new insights into the role of IL-18-based signaling in immune regulation. |
| WOS关键词 | IL-1 FAMILY ; NONCANONICAL INFLAMMASOME ; IL-1-BETA SECRETION ; NLRP3 INFLAMMASOME ; GASDERMIN D ; EXPRESSION ; RECEPTOR ; BINDING ; IDENTIFICATION ; REGULATORS |
| 资助项目 | National Natural Science Foundation of China[32322085] ; National Natural Science Foundation of China[42276115] ; Youth Innovation Promotion Association CAS[2021204] ; Taishan Scholar Program of Shandong Province[2021] |
| WOS研究方向 | Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001678382600001 |
| 出版者 | SPRINGERNATURE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204682]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Jiang, Shuai |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Qingdao Agr Univ, Coll Life Sci, Qingdao 266109, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Sch Marine Sci, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yuan, Liming,Xu, Hang,Jiang, Shuai. Caspase-mediated interleukin-18 maturation promotes membrane-associated unconventional secretion and signal transduction in a teleost fish[J]. MARINE LIFE SCIENCE & TECHNOLOGY,2026:14. |
| APA | Yuan, Liming,Xu, Hang,&Jiang, Shuai.(2026).Caspase-mediated interleukin-18 maturation promotes membrane-associated unconventional secretion and signal transduction in a teleost fish.MARINE LIFE SCIENCE & TECHNOLOGY,14. |
| MLA | Yuan, Liming,et al."Caspase-mediated interleukin-18 maturation promotes membrane-associated unconventional secretion and signal transduction in a teleost fish".MARINE LIFE SCIENCE & TECHNOLOGY (2026):14. |
入库方式： OAI收割
来源：海洋研究所
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