Investigation of monosaccharide colorimetric efficiency by phenol-sulfuric acid method and optimization of quantitative models for polysaccharides
文献类型：期刊论文
| 作者 | Li, Anbang2,3,4; Liu, Chang1,2; Xing, Ronge2,3; Liu, Song2,3; Li, Kecheng2,3 |
| 刊名 | CARBOHYDRATE RESEARCH
 |
| 出版日期 | 2026-04-01 |
| 卷号 | 562页码:10 |
| 关键词 | Phenol-sulfuric acid method Monosaccharides colorimetric efficiency Quantitative models Quantification of polysaccharides |
| ISSN号 | 0008-6215 |
| DOI | 10.1016/j.carres.2026.109835 |
| 通讯作者 | Li, Kecheng(lkc@qdio.ac.cn) |
| 英文摘要 | Phenol-sulfuric acid method, a classical approach for total sugar quantification, suffers from substantial variation in the colorimetric efficiency among different monosaccharides, which may result in significant measurement bias. In this study, we investigated the colorimetric efficiency of 14 monosaccharides across three different systems and found that the colorimetric efficiency followed the pattern: neutral > acidic > amino monosaccharides. Notably, the colorimetric efficiency of mannose was over 800-fold higher than that of glucosamine. Principal coordinate analysis (PCoA) revealed that functional groups (carboxyl, amino) were the major determinants of colorimetric efficiency. Based on these findings, we assessed three quantitative models for five polysaccharides. The traditional model was suitable for homogeneous glucans (e.g., starch, recovery similar to 101.0 %). The dominant monosaccharide model was effective for neutral polysaccharides with a single monosaccharide composition (e.g., mannan, recovery similar to 100.5 % in the optimized system). In contrast, the weighted model, which integrates molar ratios and individual efficiencies, failed to improve accuracy for complex heterogeneous acidic polysaccharides (e.g., fucoidans, recovery <40.0 %). Furthermore, amino groups in polysaccharides (e.g., chitosan) strongly inhibited the reaction, precluding accurate quantification. This study provides a theoretical basis for the precise application of the phenol-sulfuric acid method and offers methodological guidance for the standardization of polysaccharide analysis. |
| WOS关键词 | RAPID-DETERMINATION ; CARBOHYDRATE ; ANTIOXIDANT |
| 资助项目 | National Key Research and Development Program of China[2023YFD2401105] ; National Natural Science Foundation of China[42576119] ; National Natural Science Foundation of China[42276097] ; Natural Science Foundation of Shandong Province[ZR2022YQ37] ; Shandong Key R & D Plan, Major Scientific and Technological Innovation Project[2022CXGC020413] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001681053600001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204706]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Kecheng |
| 作者单位 | 1.Qingdao Agr Univ, Marine Sci & Engn Coll, Qingdao 266109, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Anbang,Liu, Chang,Xing, Ronge,et al. Investigation of monosaccharide colorimetric efficiency by phenol-sulfuric acid method and optimization of quantitative models for polysaccharides[J]. CARBOHYDRATE RESEARCH,2026,562:10. |
| APA | Li, Anbang,Liu, Chang,Xing, Ronge,Liu, Song,&Li, Kecheng.(2026).Investigation of monosaccharide colorimetric efficiency by phenol-sulfuric acid method and optimization of quantitative models for polysaccharides.CARBOHYDRATE RESEARCH,562,10. |
| MLA | Li, Anbang,et al."Investigation of monosaccharide colorimetric efficiency by phenol-sulfuric acid method and optimization of quantitative models for polysaccharides".CARBOHYDRATE RESEARCH 562(2026):10. |
入库方式： OAI收割
来源：海洋研究所
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