中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
  4. 实验海洋生物学重点实验室
Edwardsiella tarda infection induces PANoptosis in gill cells of Japanese flounder Paralichthys olivaceus

文献类型：期刊论文

作者Sun, Chunyan1,2,3; Jiang, Shuai1,3,4
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2026-07-01
卷号174页码:10
关键词Edwardsiella tarda PANoptosis MAPK NF-& kcy B
ISSN号1050-4648
DOI10.1016/j.fsi.2026.111316
通讯作者Jiang, Shuai(sjiang@qdio.ac.cn)
英文摘要Edwardsiella tarda is an important bacterial pathogen in fish aquaculture, yet the molecular mechanisms underlying infection remain largely unexplored. Here, we show that E. tarda infection induces rapid cell death in flounder gill cells in a dose- and time-dependent manner. Infected cells display severe plasma membrane disruption and loss of viability, consistent with necrotic death. Gene expression analysis revealed strong upregulation of immune-related genes, particularly those involved in pattern recognition receptors (TLR, NLR and RIG-I) and their intracellular downstream NF-& kcy;B and MAPK signaling pathways that control inflammation and cell survival. Blocking either the NF-& kcy;B or MAPK signaling pathways markedly reduced cell death, indicating that host signaling actively contributes to infection-induced cell death. Time-lapse live-cell imaging and biochemical analyses further demonstrated that infection triggers PANoptosis, a coordinated form of lytic cell death combining pyroptosis, apoptosis, and necrosis. Inhibition of the NF-& kcy;B pathway increased expression of proinflammatory cytokines TNF-alpha and IL-1 beta, and significantly reduced bacterial burden in flounder tissues. These findings collectively reveal a previously unrecognized molecular mechanism underlying E. tarda infectioninduced cell lysis, and highlight an important role of the NF-& kcy;B/MAPK signaling axis in fish inflammatory immune response and antimicrobial defense.
WOS关键词NF-KAPPA-B ; EXPRESSION ; PYROPTOSIS ; DEATH ; IDENTIFICATION ; MECHANISMS ; VIRULENCE ; SYSTEM
资助项目National Natural Science Foundation of China[32322085] ; National Natural Science Foundation of China[42276115] ; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2020Q03] ; Youth Innovation Promotion Association CAS[2021204] ; Taishan Scholar Program of Shandong Province[2021]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:001740234300001
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
源URL[http://ir.qdio.ac.cn/handle/337002/204895]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Jiang, Shuai
作者单位1.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China
2.Qingdao Agr Univ, Coll Life Sci, Qingdao, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266000, Peoples R China
4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Chunyan,Jiang, Shuai. Edwardsiella tarda infection induces PANoptosis in gill cells of Japanese flounder Paralichthys olivaceus[J]. FISH & SHELLFISH IMMUNOLOGY,2026,174:10.
APA Sun, Chunyan,&Jiang, Shuai.(2026).Edwardsiella tarda infection induces PANoptosis in gill cells of Japanese flounder Paralichthys olivaceus.FISH & SHELLFISH IMMUNOLOGY,174,10.
MLA Sun, Chunyan,et al."Edwardsiella tarda infection induces PANoptosis in gill cells of Japanese flounder Paralichthys olivaceus".FISH & SHELLFISH IMMUNOLOGY 174(2026):10.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。