Enzymatic depolymerization, structural characterization, and anticoagulant activity evaluation of chondroitin sulfate A oligosaccharides
文献类型：期刊论文
| 作者 | Li, Kaiqiang1,2; Liu, Yuanjie2; Zhang, Chen1,2; Yu, Huahua2,3; Liu, Song2,3; Xing, Ronge2,3; Li, Pengcheng2,3; Li, Rongfeng2,3 |
| 刊名 | CARBOHYDRATE RESEARCH
 |
| 出版日期 | 2026-06-01 |
| 卷号 | 564页码:10 |
| 关键词 | Chondroitin sulfate A Oligosaccharides Anticoagulant activity Structure-activity relationship |
| ISSN号 | 0008-6215 |
| DOI | 10.1016/j.carres.2026.109904 |
| 通讯作者 | Li, Rongfeng(rongfengli@qdio.ac.cn) |
| 英文摘要 | Chondroitin sulfate (CS) polysaccharides exhibit intrinsic structural heterogeneity, limiting precise studies of their bioactivities and therapeutic applications. To obtain homogeneous CS oligosaccharides and elucidate structure-activity relationships, we developed a controlled enzymatic method for preparing well-defined chondroitin sulfate A (CSA) oligosaccharides and investigated their chain length-dependent anticoagulant mechanisms. Herein, hyaluronidase was used to depolymerize CSA and subsequently purified by sequential ultrafiltration and anion-exchange chromatography, yielding CSA oligosaccharide fractions (CSA4, CSA6, CSA8, CSA10 and CSA12). Their structures were confirmed by NMR spectroscopy and mass spectrometry. In vitro anticoagulation assays showed significant chain-length dependence: longer oligomers (CSA8, CSA10 and CSA12) profoundly prolonged activated partial thromboplastin time (APTT) and thrombin time (TT), suggesting potentiation of endogenous serine protease inhibitors. However, shorter oligomers (CSA4 and CSA6) just selectively prolonged prothrombin time (PT), indicating extrinsic pathway modulation. This work provides a reliable method for preparing CSA oligosaccharides and establishes a molecular foundation for developing safer, predictable CS-based anticoagulants. |
| 资助项目 | Natural Science Foundation of Nantong Municipality[JC2023031] ; Qingdao West Coast New District University Presi-dents Fund |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001735221300001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204943]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Rongfeng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Kaiqiang,Liu, Yuanjie,Zhang, Chen,et al. Enzymatic depolymerization, structural characterization, and anticoagulant activity evaluation of chondroitin sulfate A oligosaccharides[J]. CARBOHYDRATE RESEARCH,2026,564:10. |
| APA | Li, Kaiqiang.,Liu, Yuanjie.,Zhang, Chen.,Yu, Huahua.,Liu, Song.,...&Li, Rongfeng.(2026).Enzymatic depolymerization, structural characterization, and anticoagulant activity evaluation of chondroitin sulfate A oligosaccharides.CARBOHYDRATE RESEARCH,564,10. |
| MLA | Li, Kaiqiang,et al."Enzymatic depolymerization, structural characterization, and anticoagulant activity evaluation of chondroitin sulfate A oligosaccharides".CARBOHYDRATE RESEARCH 564(2026):10. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。