Phage-based suppression of plasmid-mediated antibiotic resistance: A promising approach for antimicrobial stewardship
文献类型：期刊论文
| 作者 | Wang, Yan-Zi1,2,8; Li, Hu7,8; Xue, Bei-Ning6,7; Zhao, Xiao-Xu1,2; Rao, Yu1,2; Zhao, Sha7,8; Fortuna, Kiandro J.5; Ye, Mao4,7; Su, Jian-Qiang3,7,8 |
| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
 |
| 出版日期 | 2026-05-01 |
| 卷号 | 508页码:13 |
| 关键词 | Bacteriophages Antibiotic resistance genes (ARGs) Antibiotic resistant bacteria (ARB) Plasmid-dependent phages (PDPs) Biocontrol |
| ISSN号 | 0304-3894 |
| DOI | 10.1016/j.jhazmat.2026.141936 |
| 通讯作者 | Su, Jian-Qiang(jqsu@iue.ac.cn) |
| 英文摘要 | The global spread of antibiotic resistant bacteria (ARB), particularly plasmid-bearing strains, poses a major threat to public health due to their association with life-threatening infections. As alternatives to conventional antibiotics become increasingly necessary, bacteriophages are recognized as promising tools for controlling ARB. This study recovered three lytic phages, vB_EcoP_LHP, vB_EcoP_IUE, and vB_EcoM_BL from agricultural soil, which specifically target Escherichia coli K12 carrying the multidrug-resistant plasmid RP4. Based on morphological and phylogenetic analyses, phage LHP was classified within the genus Cronosvirus, subfamily Melnykvirinae, phage BL within the species Felixounavirus JLBYU32, genus Felixounavirus, subfamily Ounavirinae, while phage IUE was identified as a novel Caudoviricetes phage. No virulence, antibiotic resistance, or lysogenyassociated genes were detected, supporting their biosafety for application. Phages LHP and IUE exhibited strong lytic activity against E. coli strains carrying RP4 and pKJK5, with optimal multiplicity of infection of 100 and estimated burst sizes of 40 and 600 PFU/cell, respectively, but were more sensitive to high temperatures. In contrast, phage BL displayed a broader host range, an estimated burst size of approximately 45 PFU/cell, and higher thermal stability. In soil microcosms, substantial reductions in E. coli K12 (RP4) and plasmid persistence were achieved through phage treatment, with the cocktail showing the most rapid and sustained suppression. Notably, durable inhibition was conferred by the phage IUE, whereas moderate effects were exerted by phages LHP and BL. Collectively, these findings highlight the potential of strictly lytic putative plasmid-dependent phages, particularly in cocktails, as eco-friendly biocontrol agents for constraining the environmental dissemination of plasmid-mediated antibiotic resistance. Synopsis: This study isolated and characterized three novel lytic phages and demonstrated their potential, individually and in cocktails, to control soil-borne antibiotic resistant bacteria and plasmid-mediated resistance. |
| WOS关键词 | SOIL ; THERAPY ; BACTERIA ; HOST ; MULTIPLE ; SEQUENCE ; GENES ; DNA |
| 资助项目 | National Natural Science Foundation of China[42507186] ; National Key R & D Program of China[2024YFD1700702] ; Fujian Provincial Natural Science Foundation of China[2025J011029] ; Startup Fund for Advanced Talents of Putian University[2024164] ; Open Research Subject of Zhejiang Key Laboratory of Pollution Control for Port-Petrochemical Industry[Y202511] |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001737777500001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204958]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Su, Jian-Qiang |
| 作者单位 | 1.Putian Univ, Fujian Prov Univ, Key Lab Ecol Environm & Informat Atlas, Putian 351100, Peoples R China 2.Putian Univ, Coll Environm & Biol Engn, Fujian Key Lab Ecol Impacts & Treatment Technol Em, Putian 351100, Peoples R China 3.CAS Haixi Ind Technol Innovat Ctr Beilun, Zhejiang Key Lab Urban Environm Proc & Pollut Cont, Ningbo 315830, Peoples R China 4.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Peoples R China 5.Katholieke Univ Leuven, Belgium Leuven Plant Inst, Dept Biosyst, Leuven, Belgium 6.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 7.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 8.Chinese Acad Sci, Inst Urban Environm, State Key Lab Reg & Urban Ecol, Ningbo Observat & Res Stn, Xiamen 361021, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yan-Zi,Li, Hu,Xue, Bei-Ning,et al. Phage-based suppression of plasmid-mediated antibiotic resistance: A promising approach for antimicrobial stewardship[J]. JOURNAL OF HAZARDOUS MATERIALS,2026,508:13. |
| APA | Wang, Yan-Zi.,Li, Hu.,Xue, Bei-Ning.,Zhao, Xiao-Xu.,Rao, Yu.,...&Su, Jian-Qiang.(2026).Phage-based suppression of plasmid-mediated antibiotic resistance: A promising approach for antimicrobial stewardship.JOURNAL OF HAZARDOUS MATERIALS,508,13. |
| MLA | Wang, Yan-Zi,et al."Phage-based suppression of plasmid-mediated antibiotic resistance: A promising approach for antimicrobial stewardship".JOURNAL OF HAZARDOUS MATERIALS 508(2026):13. |
入库方式： OAI收割
来源：海洋研究所
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