Solubilization of crystalline chitin with a single LPMO: generating chito-oligosaccharides with unprecedented bioactivity
文献类型：期刊论文
| 作者 | Li, Kecheng2,3,4; Kidibule, Peter E.3; Li, Anbang1,2,4; Zhu, Siqi1,2,4; Golten, Ole3; Tuveng, Tina Rise3; Eijsink, Vincent G. H.3 |
| 刊名 | GREEN CHEMISTRY
 |
| 出版日期 | 2026-03-13 |
| 页码 | 14 |
| ISSN号 | 1463-9262 |
| DOI | 10.1039/d6gc00449k |
| 通讯作者 | Eijsink, Vincent G. H.(vincent.eijsink@nmbu.no) |
| 英文摘要 | Chitin is the second most abundant biopolymer in nature after cellulose and is very recalcitrant. The most valuable chitin-derived products are soluble polymeric chitosan and oligomeric chito-oligosaccharides, the production of which involves environmentally unfriendly processing steps with concentrated acid and alkali. Enzymatic valorization of chitin has not been explored industrially, and enzymatic routes from chitin to soluble oligomeric or polymeric products are lacking. Lytic polysaccharide monooxygenases (LPMOs) contribute to chitin turnover in nature because they promote the activity of chitinases. Here, we have explored whether such LPMOs can be used as stand-alone enzymes to solubilize chitin, yielding, in a single enzymatic step, bioactive oxidized chito-oligosaccharides (oxCHOS). We show that, indeed, chitin can be solubilized using only an LPMO, reaching up to 34% solubilization with a single enzyme treatment and 60% after four consecutive treatments. Importantly, the resulting soluble oxCHOS, with a degree of polymerization of 4-10, showed much stronger immunostimulatory activity on murine macrophages compared to non-oxidized CHOS. We also show that further diversification of the resulting oxCHOS may be achieved by subsequent treatment with chitin deacetylases. These results are poised to reshape current perceptions regarding the valorization of chitin biomass, opening a new green processing route, directly from chitin to products with high bioactivity. |
| WOS关键词 | LYTIC POLYSACCHARIDE MONOOXYGENASE ; ENZYMATIC PRODUCTION ; SERRATIA-MARCESCENS ; IMMUNITY ; BINDING ; DEGRADATION ; CONVERSION ; ENZYMES |
| 资助项目 | Chinese Academy of Sciences[058GJHZ2023044FN] ; Norges Forskningsrd[353185] ; Natural Science Foundation of Shandong Province[ZR2022YQ37] ; National Natural Science Foundation of China[42576119] ; National Natural Science Foundation of China[42276097] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49] ; European Commission[101059786] |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001728610000001 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/205033]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Eijsink, Vincent G. H. |
| 作者单位 | 1.Univ Chinese Acad Sci, Marine Sci Coll, Beijing 101408, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China 3.Norwegian Univ Life Sci NMBU, Fac Chem Biotechnol & Food Sci, N-1432 As, Norway 4.Chinese Acad Sci, Key Lab Expt Marine Biol, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Kecheng,Kidibule, Peter E.,Li, Anbang,et al. Solubilization of crystalline chitin with a single LPMO: generating chito-oligosaccharides with unprecedented bioactivity[J]. GREEN CHEMISTRY,2026:14. |
| APA | Li, Kecheng.,Kidibule, Peter E..,Li, Anbang.,Zhu, Siqi.,Golten, Ole.,...&Eijsink, Vincent G. H..(2026).Solubilization of crystalline chitin with a single LPMO: generating chito-oligosaccharides with unprecedented bioactivity.GREEN CHEMISTRY,14. |
| MLA | Li, Kecheng,et al."Solubilization of crystalline chitin with a single LPMO: generating chito-oligosaccharides with unprecedented bioactivity".GREEN CHEMISTRY (2026):14. |
入库方式： OAI收割
来源：海洋研究所
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