Artificial reef age reshapes benthic microbial communities and modulates the genetic potential for nitrogen and sulfur cycling
文献类型：期刊论文
| 作者 | Wang, Shen-Tong1,3,4; Li, Li1,2,3,4,6; Yang, Qi4; Zhang, Guo-Fan5,6 |
| 刊名 | ENVIRONMENTAL RESEARCH
 |
| 出版日期 | 2026-06-15 |
| 卷号 | 299页码:13 |
| 关键词 | Artificial reef Metagenomics Microbiome Nitrogen Reef age Sulfur |
| ISSN号 | 0013-9351 |
| DOI | 10.1016/j.envres.2026.124314 |
| 通讯作者 | Li, Li(lili@qdio.ac.cn) |
| 英文摘要 | Artificial reefs (ARs) are widely used to restore coastal ecosystems; however, the impact of reef age on microbial communities and their biogeochemical functions remains unknown. This study integrated metagenomic sequencing with physicochemical analysis to examine successional changes in benthic nitrogen and sulfur cycling along a chronosequence spanning from non-artificial reefs (0 years) to 14-year-old ARs in the coastal waters of the Bohai Sea, China. Our analysis revealed a systematic, time-dependent reorganization of the benthic micro-biome, characterized by significant enrichment of ammonia-oxidizing archaea (Nitrososphaerota) and bacteria (Nitrospirota) in reefs older than 6 years. Conversely, taxa involved in coupled nitrate reduction and sulfur oxidation (Sulfurovum) declined significantly. Functionally, this led to a shift in genetic potential: the abundance of nitrification genes (amoB and amoC) increased, while genes associated with dissimilatory nitrate reduction (nirB and nrfA), denitrification (nosZ and napB), thiosulfate reduction (phsC and ttrB), and sulfur oxidation (sqr and sox) decreased. Genome-resolved analysis further demonstrated that these functional shifts were driven by the proliferation of nitrifiers and concurrent decline of versatile bacterial lineages. Importantly, this genomic shift was corroborated by geochemical observations of decreased ammonium and increased nitrate concentrations in both bottom seawater and sediments of ARs compared to non-artificial reefs. These results indicate that reef age reshapes benthic microbial communities and functions, favoring aerobic nitrification over anaerobic or microaerophilic nitrate reduction and sulfur metabolism. This study provides a scientific basis for AR adaptive management, underscoring the necessity of integrating microbial functional metrics into the long-term impact assessment of marine infrastructures. |
| WOS关键词 | NITROSPIRA ; DIVERSITY ; ALGORITHM ; BACTERIA ; GENOMES ; FRESH |
| 资助项目 | Key R & D Program of Shandong Province[ZFJH202309] ; Key R & D Program of Shandong Province[2025TZXD030] ; National Key R & D Program of China[2025YFD2400600] ; National Key R & D Program of China[2023YFD2401102] ; Department of Science & Technology of Shandong Province[2022GJJLJRC02-096] ; China Agriculture Research System of MOF and MARA[CARS-49] |
| WOS研究方向 | Environmental Sciences & Ecology ; Public, Environmental & Occupational Health |
| 语种 | 英语 |
| WOS记录号 | WOS:001723661000001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/205154]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Li |
| 作者单位 | 1.Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao 266071, Peoples R China 2.Shandong Ctr Technol Innovat Oyster Seed Ind, Qingdao 266105, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao 266000, Shandong, Peoples R China 5.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266000, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Shen-Tong,Li, Li,Yang, Qi,et al. Artificial reef age reshapes benthic microbial communities and modulates the genetic potential for nitrogen and sulfur cycling[J]. ENVIRONMENTAL RESEARCH,2026,299:13. |
| APA | Wang, Shen-Tong,Li, Li,Yang, Qi,&Zhang, Guo-Fan.(2026).Artificial reef age reshapes benthic microbial communities and modulates the genetic potential for nitrogen and sulfur cycling.ENVIRONMENTAL RESEARCH,299,13. |
| MLA | Wang, Shen-Tong,et al."Artificial reef age reshapes benthic microbial communities and modulates the genetic potential for nitrogen and sulfur cycling".ENVIRONMENTAL RESEARCH 299(2026):13. |
入库方式： OAI收割
来源：海洋研究所
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