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Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection

文献类型：期刊论文

作者Ding, Xin1,2,5; Chen, Yuan4; Wang, Qingyue1,2,5; Yuan, Zihao1,2,3,5; Zhang, Jian4; Li, Xuepeng4; Xu, Hang2,5; Sun, Li1,2,3,5
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2026-06-01
卷号173页码:12
关键词Lateolabrax maculatus Gasdermin Pyroptosis Caspase Photobacterium damselae
ISSN号1050-4648
DOI10.1016/j.fsi.2026.111286
通讯作者Xu, Hang(xuhang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
英文摘要Pyroptosis is a lytic form of programmed cell death executed by gasdermin, which is activated by caspase (CASP) cleavage. Pyroptosis plays a pivotal role in host defense against bacterial infection. Spotted seabass Lateolabrax maculatus, one of the major aquaculture fish in China, is vulnerable to the bacterial pathogen Photobacterium damselae subsp. piscicida (PDP), which causes significant economic losses due to high mortality outbreaks. To date, no study on L. maculatus gasdermin has been documented, and the role of gasdermin in bacterial infection is unclear. In this study, we identified from L. maculatus two gasdermin E (GSDME) (designated LmGSDMEa and LmGSDMEb, respectively) and three CASPs (designated LmCASP3, 6, and 7, respectively), and characterized their biological activities. LmCASP3 and LmCASP7, but not LmCASP6, directly induced apoptosis. LmCASP3/7 specifically cleaved LmGSDMEa at the 262NTVD265 site, generating an active N-terminal fragment (NT265) that converted apoptosis into pyroptosis. Unlike LmGSDMEa, LmGSDMEb could not trigger pyroptosis. When spotted seabass were challenged with PDP, the expression levels of LmCASP3/7, LmGSDMEa, and pyroptosis-associated inflammatory cytokines were upregulated in fish tissues. Consistently, PDP infection promoted the production and activation of LmGSDMEa and LmCASP3/7 in spotted seabass macrophages, resulting in pyroptotic cell death. Collectively, this study revealed the activation and regulation mechanism of GSDMEa-mediated pyroptosis in spotted seabass, and provided new insights into the role of pyroptosis in fish immunity during bacterial infection.
WOS关键词CELL-DEATH ; INFLAMMATORY CASPASES ; NLRP3 INFLAMMASOME ; CANCER CELLS ; GASDERMIN D ; GENE ; PROTEIN ; EXPRESSION ; APOPTOSIS ; CLEAVAGE
资助项目Joint Fund of the National Natural Science Foundation of China[U24A20463] ; Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203000] ; National Key Research and Development Project of China[2018YFD0900500]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:001724450800001
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
源URL[http://ir.qdio.ac.cn/handle/337002/205163]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xu, Hang; Sun, Li
作者单位1.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao, Peoples R China
2.Chinese Acad Sci, Inst Oceanol, Lab Expt Marine Biol, Qingdao, Peoples R China
3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Yantai Univ, Sch Ocean, Yantai, Peoples R China
5.Chinese Acad Sci, State Key Lab Breeding Biotechnol & Sustainable Aq, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Ding, Xin,Chen, Yuan,Wang, Qingyue,et al. Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection[J]. FISH & SHELLFISH IMMUNOLOGY,2026,173:12.
APA Ding, Xin.,Chen, Yuan.,Wang, Qingyue.,Yuan, Zihao.,Zhang, Jian.,...&Sun, Li.(2026).Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection.FISH & SHELLFISH IMMUNOLOGY,173,12.
MLA Ding, Xin,et al."Spotted seabass Lateolabrax maculatus GSDMEa: a pyroptosis executor activated by CASP3/7 during bacterial infection".FISH & SHELLFISH IMMUNOLOGY 173(2026):12.

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来源：海洋研究所

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