In Situ Harvesting and Molecular Identification for the Germinating Species Diversity of Dinoflagellate Resting Cysts in Jiaozhou Bay, China
文献类型：期刊论文
| 作者 | Shi, Shuo4,5; Yang, Wanli4,5; Tao, Zhe4,6; Li, Fengting1,4; Wei, Ben2,4; Yue, Caixia3,4; Deng, Yunyan1,4; Shang, Lixia1,4; Chai, Zhaoyang1,4; Tang, Ying-Zhong1,4 |
| 刊名 | LIFE-BASEL
 |
| 出版日期 | 2025-10-27 |
| 卷号 | 15期号:11页码:21 |
| 关键词 | in situ monitoring of germination dinoflagellate resting cyst Germlings Harvester (GEHA) HABs |
| DOI | 10.3390/life15111670 |
| 通讯作者 | Chai, Zhaoyang(zhaoyangchai@qdio.ac.cn) ; Tang, Ying-Zhong(yingzhong.tang@qdio.ac.cn) |
| 英文摘要 | Dinoflagellate resting cysts are critical to dinoflagellate ecology, acting as a key seed source for initiating harmful algal blooms (HABs) through their germination. However, the in situ germination dynamics of these cysts remain poorly understood due to technical challenges. To overcome this, we utilized the Germlings Harvester (GEHA), an in situ germination device we designed, to collect water samples containing dinoflagellate cysts germinated from marine sediments in Jiaozhou Bay, China, after 5 and 20 days of incubation. By combining the GEHA with metabarcoding analysis targeting 28S rDNA-specific primers for dinoflagellates, we identified 44 dinoflagellate species spanning 31 genera, 18 families, and 7 orders. Of these, 12 species were linked to HABs or recognized as toxic, including Azadinium poporum, Alexandrium leei, Alexandrium pacificum, Akashiwo sanguinea, Karlodinium veneficum, Stoeckeria algicida, and Luciella masanensis. Additionally, five species were newly identified as cyst producers, and one symbiotic dinoflagellate, Effrenium voratum, was detected. Our results also found that germinated dinoflagellate species increased from 23 to 34 with extended incubation, and the ratio of mixotrophic to heterotrophic species was approximately 2:1 in the samples of in situ sediments and seawater outside GEHA, as well as across germination durations (Sg-5 d vs. Sg-20 d). These findings provide essential field evidence for the role of resting cysts in driving HAB formation in this region and highlight the efficacy of the GEHA-based approach for studying in situ cyst germination dynamics, offering a robust tool for monitoring, early warning, prevention, and forecasting of HABs. |
| WOS关键词 | HARMFUL ALGAL BLOOMS ; SP-NOV DINOPHYCEAE ; COASTAL WATERS ; KARLODINIUM-VENEFICUM ; GYRODINIUM-INSTRIATUM ; STOECKERIA-ALGICIDA ; SCRIPPSIELLA DINOPHYCEAE ; TAXONOMIC CLARIFICATION ; PHYLOGENETIC POSITION ; ALEXANDRIUM-CATENELLA |
| 资助项目 | Shandong Provincial Natural Science Foundation[ZR2024MD109] ; the National Key R&D Program of China[2024YFF0506901] ; the Key Research Infrastructures in the CAS Field Stations of the Chinese Academy of Science[KFJ-SWYW047] ; the National Science Foundation of China[42406210] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001624697800001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204268]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chai, Zhaoyang; Tang, Ying-Zhong |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, China Lab Marine Ecol & Environm Sci, Qingdao 266237, Peoples R China 2.Harbin Engn Univ, Qingdao Innovat & Dev Ctr, Qingdao 266000, Peoples R China 3.Anim Plant & Food Inspect Ctr Nanjing Customs, Nanjing 210000, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Shandong Marine Resource & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Shuo,Yang, Wanli,Tao, Zhe,et al. In Situ Harvesting and Molecular Identification for the Germinating Species Diversity of Dinoflagellate Resting Cysts in Jiaozhou Bay, China[J]. LIFE-BASEL,2025,15(11):21. |
| APA | Shi, Shuo.,Yang, Wanli.,Tao, Zhe.,Li, Fengting.,Wei, Ben.,...&Tang, Ying-Zhong.(2025).In Situ Harvesting and Molecular Identification for the Germinating Species Diversity of Dinoflagellate Resting Cysts in Jiaozhou Bay, China.LIFE-BASEL,15(11),21. |
| MLA | Shi, Shuo,et al."In Situ Harvesting and Molecular Identification for the Germinating Species Diversity of Dinoflagellate Resting Cysts in Jiaozhou Bay, China".LIFE-BASEL 15.11(2025):21. |
入库方式： OAI收割
来源：海洋研究所
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