Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of the toxigenic harmful algae bloom species Pseudo-nitzschia multistriata
文献类型：期刊论文
| 作者 | Wang, Hui1,3,4,5; Chen, Nansheng1,2,3,5 |
| 刊名 | JOURNAL OF APPLIED PHYCOLOGY
 |
| 出版日期 | 2026-02-07 |
| 页码 | 16 |
| 关键词 | |
| ISSN号 | 0921-8971 |
| DOI | 10.1007/s10811-025-03766-9 |
| 通讯作者 | Chen, Nansheng(chenn@qdio.ac.cn) |
| 英文摘要 | The diatom genus Pseudo-nitzschia includes many species that are cosmopolitan and can cause toxigenic harmful algal blooms (HABs). These blooms are capable of producing domoic acid (DA), a kainic acid-type neurotoxin that causes amnesic shellfish poisoning (ASP), which poses threats to marine animals and humans. It is thus important to develop rapid technologies that enable monitoring of toxigenic Pseudo-nitzschia species with high specificity and sensitivity to facilitate early warning. In this study, we developed a rapid assay for the toxigenic species P. multistriata, based on its dabA gene, which has been demonstrated to be a key gene in DA biosynthesis, combining recombinase polymerase amplification with lateral flow dipstick (RPA-LFD). The RPA-LFD assay is specific to P. multistriata and has high sensitivity, with a detection limit of 0.02 ng mu L-1 for genomic DNA, a cell density detection limit of approximately 1 cells/reaction, and a detection limit of 3.36 x 101 copies mu L-1 for recombinant plasmid DNA. The established RPA-LFD assay can complete the reaction in just 30 min at 37 degrees C, and the results can be directly visualized on the LFD within a short period of time, typically within 10 min. The practicality of this assay for on-site detection has also been verified in this study. The RPA-LFD assay with the high specific and sensitivity developed in this study will aid in predicting toxigenic P. multistriata blooms. |
| WOS关键词 | DOMOIC ACID ; MICROARRAY ; PROBES ; BACILLARIOPHYCEAE ; DIATOM ; QUANTIFICATION ; IDENTIFICATION ; DIAGNOSIS ; SHELLFISH ; AUSTRALIS |
| 资助项目 | the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[LSKJ202203700] ; the National Key Research and Development (R&D) Program of China[No.2022YFC3105200] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001682888300001 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204709]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, Nansheng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 100039, Peoples R China 5.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hui,Chen, Nansheng. Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of the toxigenic harmful algae bloom species Pseudo-nitzschia multistriata[J]. JOURNAL OF APPLIED PHYCOLOGY,2026:16. |
| APA | Wang, Hui,&Chen, Nansheng.(2026).Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of the toxigenic harmful algae bloom species Pseudo-nitzschia multistriata.JOURNAL OF APPLIED PHYCOLOGY,16. |
| MLA | Wang, Hui,et al."Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid and sensitive detection of the toxigenic harmful algae bloom species Pseudo-nitzschia multistriata".JOURNAL OF APPLIED PHYCOLOGY (2026):16. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。