Cyst induction by intracellular Mg2+/Ca2+ injection and its implications for optimizing Mg2+ conditions for future CRISPR delivery in Alexandrium catenella
文献类型：期刊论文
| 作者 | Chen, Zhenfan3; Guo, Chang2,3; Geng, Huixia1,3; Kong, Fanzhou1,3; Zhang, Qingchun1,3; Yu, Rencheng1,2,3 |
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2026-04-01 |
| 页码 | 10 |
| 关键词 | dinoflagellate
|
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-026-5157-x |
| 通讯作者 | Yu, Rencheng(rcyu@qdio.ac.cn) |
| 英文摘要 | The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas system is a powerful tool for gene editing, yet it has never been successfully applied to edit genes in dinoflagellates. In CRISPR-Cas-mediated gene editing, magnesium ions (Mg2+) are necessary to maintain catalytic activity. Some studies, however, found that Mg2+ and calcium (Ca2+) ions could modulate flagellar motility and cyst formation of unicellular flagellates, thus affecting their physiology. Here, a single-cell microinjection method was adapted to deliver Mg2+/Ca2+ into cells of the dinoflagellate Alexandrium catenella to test the dose-dependent effects on cyst formation and germination. It was found that Mg2+/Ca2+ treatments <= 50 mmol/L significantly enhanced cyst survival and germination rates on day 1 compared to those of higher concentrations. In contrast, treatments with high concentrations (<= 250 mmol/L) of Mg2+/Ca2+ triggered irreversible dormancy transitions, accompanied by increased mortality and prolonged germination time. The treatment with 10-mmol/L Mg2+ achieved an optimal balance between CRISPR-Cas nuclease activity and cyst germination. This work provides the first experimental assessment of ion, induced cyst physiology using single-cell microinjection in dinoflagellates, and identifies Mg2+ concentrations that are physiologically compatible with microinjection. These findings establish the fundamental conditions for future microinjection-based CRISPR delivery in A. catenella. |
| WOS关键词 | DINOPHYCEAE ; GERMINATION ; EUGLENA ; GULF ; CA2+ |
| 资助项目 | National Key R&D Programs from the Ministry of Science and Technology (MoST) of China[2022YFC3105300] ; National Key R&D Programs from the Ministry of Science and Technology (MoST) of China[2023YFE0106200] ; Taishan Scholarship of Professor Rencheng YU ; China Postdoctoral Science Foundation[2023M733523] ; Shandong Provincial Natural Science Foundation[ZR2023QD132] ; Qingdao Postdoctoral Applied Research Project[QDBSH20230202008] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001730611200001 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/205084]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Yu, Rencheng |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhenfan,Guo, Chang,Geng, Huixia,et al. Cyst induction by intracellular Mg2+/Ca2+ injection and its implications for optimizing Mg2+ conditions for future CRISPR delivery in Alexandrium catenella[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2026:10. |
| APA | Chen, Zhenfan,Guo, Chang,Geng, Huixia,Kong, Fanzhou,Zhang, Qingchun,&Yu, Rencheng.(2026).Cyst induction by intracellular Mg2+/Ca2+ injection and its implications for optimizing Mg2+ conditions for future CRISPR delivery in Alexandrium catenella.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,10. |
| MLA | Chen, Zhenfan,et al."Cyst induction by intracellular Mg2+/Ca2+ injection and its implications for optimizing Mg2+ conditions for future CRISPR delivery in Alexandrium catenella".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2026):10. |
入库方式： OAI收割
来源：海洋研究所
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