An integrated morpho-molecular approach revealed dinoflagellate cysts diversity in ballast tank sediments of foreign ships
文献类型：期刊论文
| 作者 | Yue, Caixia1,3; Tao, Zhe2,3; Chai, Zhaoyang3,6; Li, Tao5; Feng, Zhen1; Wu, Yangyun1; Hu, Zhangxi4; Liu, Yuyang7; Shi, Shuo3,6; Yang, Wanli3,6 |
| 刊名 | MARINE POLLUTION BULLETIN
 |
| 出版日期 | 2026-07-01 |
| 卷号 | 228页码:11 |
| 关键词 | Ballast tank sediments Dinoflagellate cysts Harmful algal blooms (HABs) Light microscopic observation and single-cyst PCR sequencing (LM-ScPCR sequencing) Biosecurity |
| ISSN号 | 0025-326X |
| DOI | 10.1016/j.marpolbul.2026.119568 |
| 通讯作者 | Tian, Wen(tianwen201605@163.com) ; Tang, Ying Zhong(yingzhong.tang@qdio.ac.cn) |
| 英文摘要 | Ships' ballast water has become a major vector for the global dispersal of marine organisms since 1990s. Dinoflagellate resting cysts in ballast tank sediments (BTS) pose significant ecological risks because they can survive for decades and may provide "seedlings" via germination to initiate harmful algal blooms. Despite advances in molecular technologies, diversity assessment of dinoflagellate cysts in BTS has not been firmly based on a convincing morpho-molecular approach. We combined light microscopy and single-cyst PCR sequencing (LMScPCR sequencing) together with laboratory germination to identify dinoflagellate cysts in BTS from five international vessels arriving in China. Key findings include: 1) LM-ScPCR sequencing identified 59 dinoflagellate resting cysts (40 fully identified to species level and 19 to genus level), belonging to 35 dinoflagellate species. Of these, 26 (74.3%) were fully identified as well-described species, including 5 new records for China and 9 toxic and/or HABs-causing species (e.g. Gymnodinium catenatum, Alexandrium ostenfeldii, A. insuetum). Notably, the diversity of dinoflagellate species varied among ships from different route; 2) Cyst germination experiments identified 4 dinoflagellate species (2 to species level, 2 potentially novel species), 3 of which may be harmful because other members of their con-generic species are proven toxic/harmful; and 3) Phylogenetic analyses revealed multiple unclassified taxa likely representing novel species, and novel ribo-types of species that may pose a new type of alien "species" invasion risk. Our findings further necessitate more intensive assessment and strengthened management practice in order to more effectively monitor and mitigate invasion threats. |
| WOS关键词 | ALEXANDRIUM-OSTENFELDII DINOPHYCEAE ; HARMFUL ALGAL BLOOMS ; SP-NOV DINOPHYCEAE ; RESTING CYSTS ; GYMNODINIUM-CATENATUM ; PHYLOGENETIC POSITION ; WATER ; TRANSPORT ; SEA ; IDENTIFICATION |
| 资助项目 | Research Projects of General Administration of Customs of the People's Republic of China[2025HK047] ; Research Projects of General Administration of Customs of the People's Republic of China[2024HK042] ; Jiangsu Funding Program for Excellent Postdoctoral Talent[2025ZB121] ; Research Project of Nanj-ing Customs[2025KJ25] ; National Key R & D Program of China[2024YFF0506901] ; National Natural Science Foundation of China[42406210] |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001724797200001 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/205157]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Tian, Wen; Tang, Ying Zhong |
| 作者单位 | 1.Anim Plant & Food Inspect Ctr Nanjing Customs Dist, Nanjing 210000, Peoples R China 2.Shandong Marine Resource & Environm Res Inst, Shandong Key Lab Marine Ecol Restorat, Yantai 264006, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Guangdong Ocean Univ, Coll Fisheries, Dept Aquaculture, Zhanjiang 524088, Peoples R China 5.Minist Transport, Environm Protect Ctr, Beijing 100013, Peoples R China 6.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 7.Shandong Univ Sci & Technol, Coll Safety & Environm Engn, Qingdao 266590, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yue, Caixia,Tao, Zhe,Chai, Zhaoyang,et al. An integrated morpho-molecular approach revealed dinoflagellate cysts diversity in ballast tank sediments of foreign ships[J]. MARINE POLLUTION BULLETIN,2026,228:11. |
| APA | Yue, Caixia.,Tao, Zhe.,Chai, Zhaoyang.,Li, Tao.,Feng, Zhen.,...&Tang, Ying Zhong.(2026).An integrated morpho-molecular approach revealed dinoflagellate cysts diversity in ballast tank sediments of foreign ships.MARINE POLLUTION BULLETIN,228,11. |
| MLA | Yue, Caixia,et al."An integrated morpho-molecular approach revealed dinoflagellate cysts diversity in ballast tank sediments of foreign ships".MARINE POLLUTION BULLETIN 228(2026):11. |
入库方式： OAI收割
来源：海洋研究所
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