Confocal Raman Microscopy Quantitatively Reveals Subcellular Metabolic Adaptation Mechanisms of Deep-Sea Mussels to Methane Deprivation
文献类型：期刊论文
| 作者 | He, Wanying1,6,7; Li, Mengna2,4,5,6; Wang, Minxiao4,5,6; Guo, Xiaoxiao1; Chen, Hao4,5,6; Zhong, Zhaoshan4,5,6; Xi, Shichuan1; Li, Lianfu1; Zhang, Yitong5,6,7; Zhuo, Jintao5,6,7 |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
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| 出版日期 | 2025-12-09 |
| 页码 | 12 |
| 关键词 | methane deprivation deep-sea mussel metabolicprocess quantitative analysis confocal Raman microscopy |
| ISSN号 | 0013-936X |
| DOI | 10.1021/acs.est.5c13105 |
| 通讯作者 | Zhang, Xin(xzhang@qdio.ac.cn) |
| 英文摘要 | Methane hydrates are a potential energy source, but their extraction may disrupt deep-sea ecosystems that rely on methane. Understanding biological responses to methane fluctuations in cold seep environments remains limited, primarily due to the inability to monitor subcellular metabolic dynamics in real time. This study applied confocal Raman microscopy combined with full-spectrum normalization to investigate subcellular metabolic adaptations in the gill tissues of the deep-sea mussel containing symbionts under methane deficiency. This label-free and nondestructive technique allows high-resolution quantitative analysis of biomolecules. Results demonstrate that methane deficiency induced metabolic reprogramming and structural reorganization, disrupting energy metabolism while enhancing proteolysis and lipolysis. Spatial heterogeneity is evident across cellular regions, with varying degrees of structural recovery and degradation highlighting differential resilience. These findings collectively underscore the indispensable role of methane in maintaining metabolic and structural homeostasis in deep-sea mussels, offering key insights into their adaptation strategies under methane deficiency and fundamental data for assessing the ecological impacts of methane extraction. The successful application of our methodology demonstrates broad potential for real-time metabolic monitoring in diverse organisms and for evaluating subcellular effects of environmental disturbances, establishing a new avenue for noninvasive, high-resolution quantitative metabolic tracking. |
| WOS关键词 | INFRARED-SPECTROSCOPY ; PROTEIN-STRUCTURE ; CARBON-DIOXIDE ; PHENYLALANINE ; ORIENTATION ; GLUTAMINE ; ENERGY |
| 资助项目 | Natural Science Foundation of Shandong Province[ZR2024QD074] ; National Natural Science Foundation of China[42221005] ; China Postdoctoral Science Foundation[2024M751269] |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001634073600001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204378]  |
| 专题 | 海洋研究所_海洋地质与环境重点实验室 |
| 通讯作者 | Zhang, Xin |
| 作者单位 | 1.Laoshan Lab, Qingdao 266237, Peoples R China 2.Natl Deep Sea Ctr, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou 510301, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266071, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Geol & Environm, Qingdao 266071, Peoples R China 7.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Key Lab Ocean Observat & Forecasting, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Wanying,Li, Mengna,Wang, Minxiao,et al. Confocal Raman Microscopy Quantitatively Reveals Subcellular Metabolic Adaptation Mechanisms of Deep-Sea Mussels to Methane Deprivation[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2025:12. |
| APA | He, Wanying.,Li, Mengna.,Wang, Minxiao.,Guo, Xiaoxiao.,Chen, Hao.,...&Zhang, Xin.(2025).Confocal Raman Microscopy Quantitatively Reveals Subcellular Metabolic Adaptation Mechanisms of Deep-Sea Mussels to Methane Deprivation.ENVIRONMENTAL SCIENCE & TECHNOLOGY,12. |
| MLA | He, Wanying,et al."Confocal Raman Microscopy Quantitatively Reveals Subcellular Metabolic Adaptation Mechanisms of Deep-Sea Mussels to Methane Deprivation".ENVIRONMENTAL SCIENCE & TECHNOLOGY (2025):12. |
入库方式： OAI收割
来源：海洋研究所
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