Dual detection of Escherichia coli and Staphylococcus aureus via lateral flow immunoassay sensor utilizing porous surface Ag@Au micron particles
文献类型：期刊论文
| 作者 | Zeng, Yan1; Lin, Yijun2; Chen, Jiawei1; Sun, Yan1; Qi, Peng1; Wang, Peng1 |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2026-03-01 |
| 卷号 | 450页码:12 |
| 关键词 | Lateral flow immunoassay sensor Pathogenic bacteria SERS Point-of-care testing |
| DOI | 10.1016/j.snb.2025.139264 |
| 通讯作者 | Qi, Peng(pengqi@qdio.ac.cn) ; Wang, Peng(wangpeng@qdio.ac.cn) |
| 英文摘要 | Early detection and diagnosis are essential for effectively preventing and treating complex pathogenic bacterial infections. This study presents an innovative approach for on-site, ultrasensitive pathogen detection by integrating a uniquely designed probe binding mode with novel nanomaterial structures within a lateral flow immunoassay (LFIA) platform. Through a galvanic replacement reaction, gold is non-uniformly deposited onto a silver core, resulting in the formation of Ag@pore-Au structures. The abundant nanopores and porous surface of these structures create a high density of "hot spots", significantly enhancing their surface-enhanced Raman scattering (SERS) performance. We systematically characterized the SERS and detection capabilities of the synthesized Ag@pore-Au Raman-dye micron particles (MPs) probe. The results conclusively demonstrated that this structural probe exhibits a robust SERS signal, paving the way for its successful application in LFIA. By integrating the Ag@pore-Au Raman-dye MPs probe into the LFIA platform, we achieved rapid, quantitative detection of Escherichia coli and Staphylococcus aureus. This enhanced platform overcomes the limitations of traditional LFIA, notably its high detection limit of 10 CFU/mL and its inability to provide quantitative results. Furthermore, a dual-channel detection mode enabled the simultaneous and specific identification of both bacterial strains. The platform's efficacy was validated through successful detection in real sample matrices. This research offers novel insights and methodologies for pathogen detection in the medical field, providing an effective solution for on-site pathogen diagnosis and real-time environmental monitoring. |
| 资助项目 | National Natural Science Foundation of China[42476208] ; Shandong Provincial National Science Foundation[ZR2024MD060] ; Science and technology innovation Program of Hunan Province[2024WK2010] |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:001635126200001 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204408]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Qi, Peng; Wang, Peng |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, State Key Lab Adv Marine Mat, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China 2.Power China Zhongnan Engn Corp Ltd, Changsha 410014, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Yan,Lin, Yijun,Chen, Jiawei,et al. Dual detection of Escherichia coli and Staphylococcus aureus via lateral flow immunoassay sensor utilizing porous surface Ag@Au micron particles[J]. SENSORS AND ACTUATORS B-CHEMICAL,2026,450:12. |
| APA | Zeng, Yan,Lin, Yijun,Chen, Jiawei,Sun, Yan,Qi, Peng,&Wang, Peng.(2026).Dual detection of Escherichia coli and Staphylococcus aureus via lateral flow immunoassay sensor utilizing porous surface Ag@Au micron particles.SENSORS AND ACTUATORS B-CHEMICAL,450,12. |
| MLA | Zeng, Yan,et al."Dual detection of Escherichia coli and Staphylococcus aureus via lateral flow immunoassay sensor utilizing porous surface Ag@Au micron particles".SENSORS AND ACTUATORS B-CHEMICAL 450(2026):12. |
入库方式： OAI收割
来源：海洋研究所
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