Self assembling fluorescent biosensing system based on rolling circle amplification combined with DNA templated quantum dots for detecting microbial DNA
文献类型：期刊论文
| 作者 | Wang, Xin2; Zeng, Yan3; Qi, Peng3; Wang, Peng3; Wang, Wei2; Wang, Di1 |
| 刊名 | SENSORS AND ACTUATORS B-CHEMICAL
 |
| 出版日期 | 2026-03-01 |
| 卷号 | 450页码:12 |
| 关键词 | Rolling circle amplification DNA-templated quantum dots DNA nanoflower Microbial DNA detection Fluorescent biosensing |
| DOI | 10.1016/j.snb.2025.139286 |
| 通讯作者 | Zeng, Yan(zengyan@qdio.ac.cn) ; Wang, Wei(wangwei8038@ouc.edu.cn) |
| 英文摘要 | Rapid, sensitive detection of microbial DNA is critical for public health and environmental monitoring. Existing fluorescent biosensors are often limited by complex probe preparation and lack of modularity. To overcome these challenges, we developed a universal biosensor by integrating rolling circle amplification (RCA) with DNAtemplated quantum dot (DNA-QD) self-assembly. Our approach features a one-pot synthesis of DNA-QDs with excellent fluorescence and stability, which simplifies probe production by eliminating complex modifications. In this design, RCA-generated DNA nanoflowers (DNFs) served as a robust signal amplification scaffold. This DNFs-QDs platform demonstrates a concentration-dependent fluorescence response (linear range: 1 x10-8 M to 1 x10-6 M, LOD: 1.3 x10-9 M) with high specificity, stability, and reproducibility, enabling robust quantification. Its modular design allows for universal adaptation to different DNA targets, and it functions robustly in complex environmental matrices, demonstrating high practical utility. This work delivers a sensitive, customizable, and high-performance tool with broad application potential in pathogen diagnosis and environmental monitoring. |
| WOS关键词 | CDSE |
| 资助项目 | National Nat-ural Science Foundation of China[42476208] ; National Nat-ural Science Foundation of China[42376208] ; National Nat-ural Science Foundation of China[42476210] ; National Nat-ural Science Foundation of China[ZR2024MD060] ; National Nat-ural Science Foundation of China[ZR2024ME102] ; Shandong Provincial National Science Foundation |
| WOS研究方向 | Chemistry ; Electrochemistry ; Instruments & Instrumentation |
| 语种 | 英语 |
| WOS记录号 | WOS:001638875600001 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/204421]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Zeng, Yan; Wang, Wei |
| 作者单位 | 1.Natl Inst Metrol, Ctr Adv Measurement Sci, Beijing 100029, Peoples R China 2.Ocean Univ China, Sch Mat Sci & Engn, Qingdao 266100, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, State Key Lab Adv Marine Mat, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xin,Zeng, Yan,Qi, Peng,et al. Self assembling fluorescent biosensing system based on rolling circle amplification combined with DNA templated quantum dots for detecting microbial DNA[J]. SENSORS AND ACTUATORS B-CHEMICAL,2026,450:12. |
| APA | Wang, Xin,Zeng, Yan,Qi, Peng,Wang, Peng,Wang, Wei,&Wang, Di.(2026).Self assembling fluorescent biosensing system based on rolling circle amplification combined with DNA templated quantum dots for detecting microbial DNA.SENSORS AND ACTUATORS B-CHEMICAL,450,12. |
| MLA | Wang, Xin,et al."Self assembling fluorescent biosensing system based on rolling circle amplification combined with DNA templated quantum dots for detecting microbial DNA".SENSORS AND ACTUATORS B-CHEMICAL 450(2026):12. |
入库方式： OAI收割
来源：海洋研究所
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