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Peptide nucleic acid-mediated electrochemical-photothermal dual-mode biosensor for ultrasensitive detection of sulfate-reducing bacteria

文献类型：期刊论文

作者Zhang, Shiqi2,3,4; Zhai, Xiaofan2,3,4; Yang, Jing3,4; Ma, Fubin2,3; Sun, Zhonghao3,4; Ju, Peng1; Duan, Jizhou3,4; Hou, Baorong3,4
刊名MICROCHEMICAL JOURNAL
出版日期2026-04-01
卷号223页码:9
关键词Electrochemical sensor Dual-mode biosensor Photothermal detection DsrC gene Sulfate-reducing bacteria
ISSN号0026-265X
DOI10.1016/j.microc.2026.117422
通讯作者Zhai, Xiaofan(zhaixf@qdio.ac.cn) ; Ju, Peng(jupeng@fio.org.cn)
英文摘要Sulfate-reducing bacteria (SRB) represents one of the most extensively studied microorganisms in microbiologically influenced corrosion (MIC). Developing effective, sensitive, and cost-efficient SRB detection methods is crucial for elucidating corrosion mechanisms and enabling timely corrosion monitoring. In this study, a novel dual-mode DNA biosensor was designed for the specific detection of the DsrC gene in SRB. The biosensor, constructed using a peptide nucleic acid (PNA) probe and catalytic hairpin assembly (CHA), integrated electrochemical and photothermal signal modes, resulting in superior anti-interference capacity and efficient signal amplification. Square wave voltammetry (SWV) current and temperature variations served as the signal outputs for the two modes, respectively. The biosensor achieved detection limits of 0.17 fM in the electrochemical mode and 0.66 fM in the photothermal mode, demonstrating an excellent selectivity, stability and reproducibility. Furthermore, the biosensor successfully detected the DsrC gene fragments isolated and extracted from real samples, presenting a superior practicability. Compared to existing techniques, this dual-mode biosensor offered a relatively wide detection range and low detection limits, providing a promising new approach for the sensitive and specific detection of corrosion microorganisms with significant application potential.
WOS关键词MICROBIOLOGICALLY INFLUENCED CORROSION ; NANOPARTICLES ; PROBE
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDB1210302] ; Wenhai Program of the S & T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2021WHZZB2305] ; Taishan Scholar Project of Shandong Province[tsqn202507295] ; Shandong Provincial Natural Science Foundation[ZR2025QA03] ; Shandong Provincial Natural Science Foundation[ZR2022MD023] ; Key R & D Program of Shandong Province, China[2023CXPT008] ; International Partnership Program of Chinese Academy of Sciences[058GJHZ2023058FN]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001707913100004
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/205000]  
专题海洋研究所_海洋腐蚀与防护研究发展中心
通讯作者Zhai, Xiaofan; Ju, Peng
作者单位1.Minist Nat Resources, Inst Oceanog 1, Marine Bioresource & Environm Res Ctr, Qingdao Key Lab Analyt Technol Dev & Offshore Ecoe, 6 Xianxialing Rd, Qingdao 266061, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Inst Oceanol, State Key Lab Adv Marine Mat, Lab Marine Environm Corros & Biofouling, 7 Nanhai Rd, Qingdao 266071, Peoples R China
4.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Shiqi,Zhai, Xiaofan,Yang, Jing,et al. Peptide nucleic acid-mediated electrochemical-photothermal dual-mode biosensor for ultrasensitive detection of sulfate-reducing bacteria[J]. MICROCHEMICAL JOURNAL,2026,223:9.
APA Zhang, Shiqi.,Zhai, Xiaofan.,Yang, Jing.,Ma, Fubin.,Sun, Zhonghao.,...&Hou, Baorong.(2026).Peptide nucleic acid-mediated electrochemical-photothermal dual-mode biosensor for ultrasensitive detection of sulfate-reducing bacteria.MICROCHEMICAL JOURNAL,223,9.
MLA Zhang, Shiqi,et al."Peptide nucleic acid-mediated electrochemical-photothermal dual-mode biosensor for ultrasensitive detection of sulfate-reducing bacteria".MICROCHEMICAL JOURNAL 223(2026):9.

入库方式： OAI收割

来源：海洋研究所

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