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Core-multiplex-micro component profiling for precision discrimination of multi-sources and adulterated arnebiae radix, from herbs to formulations

文献类型：期刊论文

作者Sha, Fei1,3,4; Zhang, Jianqing3,4; Wang, Cuicui3; Ou, Huiting3; Li, Yun3; Wei, Wenlong4; Yao, Changliang3; Bi, Qirui4; Ma, Xiaochi1,2; Guo, De-an1,3,4
刊名PHYTOMEDICINE
出版日期2025-09-01
卷号145页码:11
关键词Arnebiae Radix single standard to determine multi-components one-to-multiple counterfeit formulation
ISSN号0944-7113
DOI10.1016/j.phymed.2025.156954
通讯作者Zhang, Jianqing(Zhangjianqing@simm.ac.cn) ; Ma, Xiaochi(maxc1978@163.com) ; Guo, De-an(daguo@simm.ac.cn)
英文摘要Background: The "one-to-multiple" phenomenon and counterfeiting are prevalent in herbal medicines. Accurately identifying these issues in both crude herbs and commercial formulated products is crucial for ensuring drug safety and efficacy. However, this task remains inherently challenging due to the complex nature of the matrices involved. Purpose: This study aims to develop methodologies for the precise authentication of multi-sourced herbal medicines and their counterfeits, and to trace constituent species in commercial products. Methods: This paper proposes an integrated strategy termed "Core-Multiplex-Micro (CMM) component profiling for precision discrimination amid multi-sources and adulterants", using Arnebiae Radix as a case study. The strategy begins with the comparative analysis of primary component, shikonin derivatives, across two genuine species and three adulterants, employing a single standard to determine multi-components (SSDMC) approach. It then expands to include a broader range of mid- to low-abundance metabolites through fingerprinting-based classification. Subsequently, trace-level components are explored using LTQ-Orbitrap MS combined with metabolomics to authenticate the five species. Robust chemical markers are identified, validated, and translated to a cost-effective QDa MS platform for targeted detection. This tiered methodology was applied to authenticate 15 batches of commercially available raw materials and 10 batches of formulations. Results: The study successfully distinguished between two authentic Arnebiae Radix species and three adulterants simultaneously for the first time. Through cross-validation, nine diagnostic chemical markers were identified and effectively transferred from the expensive LTQ-Orbitrap MS platform to a more cost-effective QDa MS system for practical use in commercial product traceability. The research revealed that 60% of raw Arnebiae Radix materials were authentic, while 30% of the formulations matched their labeled species composition. Conclusion: This study sets a precedent for precise traceability for "one-to-multiple" and counterfeit products: tracing them from raw herbs to final formulations.
资助项目SIMM-National Engineering Research Center of TCM Standardization Technology independent research program ; State Key Laboratory of Drug Regulatory Science Project ; National Institutes for Food and Drug Control (NIFDC) , China[2024SKLDRS0101]
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种英语
WOS记录号WOS:001532745900001
出版者ELSEVIER GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/318849]  
专题中国科学院上海药物研究所
通讯作者Zhang, Jianqing; Ma, Xiaochi; Guo, De-an
作者单位1.Hangzhou Normal Univ, Sch Pharm, Hangzhou 311121, Zhejiang, Peoples R China
2.Dalian Med Univ, Affiliated Hosp 2, Pharmaceut Res Ctr, Dalian 116023, Peoples R China
3.Chinese Acad Sci, Shanghai Inst Mat Med, Haike Rd 501, Shanghai 201203, Peoples R China
4.Shanghai Univ Tradit Chinese Med, Cailun Rd 1200, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Sha, Fei,Zhang, Jianqing,Wang, Cuicui,et al. Core-multiplex-micro component profiling for precision discrimination of multi-sources and adulterated arnebiae radix, from herbs to formulations[J]. PHYTOMEDICINE,2025,145:11.
APA Sha, Fei.,Zhang, Jianqing.,Wang, Cuicui.,Ou, Huiting.,Li, Yun.,...&Guo, De-an.(2025).Core-multiplex-micro component profiling for precision discrimination of multi-sources and adulterated arnebiae radix, from herbs to formulations.PHYTOMEDICINE,145,11.
MLA Sha, Fei,et al."Core-multiplex-micro component profiling for precision discrimination of multi-sources and adulterated arnebiae radix, from herbs to formulations".PHYTOMEDICINE 145(2025):11.

入库方式： OAI收割

来源：上海药物研究所

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