Cytosolic WPRa4 and Plastoskeletal PMI4 Proteins Mediate Touch Response in a Model Organism Arabidopsis
文献类型：期刊论文
| 作者 | Wu, Kebin7; Yang, Nan7; Ren, Jia7; Liu, Shichang7; Wang, Kai7; Dai, Shuaijian7; Lu, Yinglin6; An, Yuxing6; Tian, Fuyun5; Gao, Zhaobing5 |
| 刊名 | MOLECULAR & CELLULAR PROTEOMICS
 |
| 出版日期 | 2025-07-01 |
| 卷号 | 24期号:7页码:22 |
| DOI | 10.1016/j.mcpro.2025.101015 |
| 通讯作者 | Li, Ning(boningli@ust.hk) |
| 英文摘要 | Plastoskeletal PMI4 Protein is a Key Regulator of ThigmomorphogenesisTo elucidate the early signaling components involved in thigmomorphogenesis in Arabidopsis thaliana, we combined microscopy and proximity-labeling (PL)-based quantitative biotinylproteomics to characterize the touch-responsive putative cytoskeleton-interacting protein WPRa4 (TREPH1). Our findings revealed that WPRa4 localizes near plastids and interacts with cytosolic Plastid Movement-Impaired (PMI) proteins and a plastidic translocon component, suggesting a cytoskeleton-plastid network in mechanosensing. Bioinformatic analysis of PL and cross-linking mass spectrometry (XL-MS) data identified PMI4 as a key mediator, with pmi4 mutants lacking touch-induced bolting delay, rosette size reduction, and Ca2+ oscillations. Transcriptomics further showed that PMI4 regulates touch-responsive and jasmonic acid (JA)-associated genes, such as LOX2. We propose a molecular model where interconnected Cytoskeleton-Plastoskeleton Continuum (CPC) proteins act as early mechanosensors, integrating the touch responses of plant aerial organs with calcium signaling and transcriptional reprogramming in Arabidopsis. |
| WOS关键词 | GENE-EXPRESSION ; PLASTID DIVISION ; VISUALIZATION ; MORPHOLOGY ; THALIANA ; NETWORK ; FTSZ1 ; COLD |
| 资助项目 | National Science Foundation of China[31370315] ; National Science Foundation of China[31570187] ; National Science Foundation of China[31870231] ; National Science Foundation of China[32070205] ; RGC of Hong Kong[16102422] ; RGC of Hong Kong[16103621] ; RGC of Hong Kong[16101114] ; RGC of Hong Kong[16103817] ; RGC of Hong Kong[16103615] ; RGC of Hong Kong[16100318] ; RGC of Hong Kong[16101819] ; RGC of Hong Kong[16101920] ; RGC of Hong Kong[16306919] ; RGC of Hong Kong[12103820] ; RGC of Hong Kong[R4012] ; RGC of Hong Kong[C6021-19EF] ; Innovation and Technology Commission (ITC) of Hong Kong[ITS/480/18FP] ; Innovation and Technology Commission (ITC) of Hong Kong[MHP/033/20] ; Hetao Shenzhen-Hong Kong Science and Technology Innovation Cooperation Zone project[HZQB-KCZYB-2020083] ; High-level new RD institute[2019B090904008] ; High-level Innovative Research Institute of Department of Science and Technology of Guangdong Province[2021B0909050003] ; GDAS'Project of Science and Technology Development[2022GDASZH-2022010202] ; Foreign Expert Program from the Ministry of Science and Technology of China[G2022030053L] ; HKUST[CSSET24SC01] ; National Natural Science Foundation of China[22302128] ; Guangdong Basic and Applied Basic Research Foundation[2024A1515010990] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001530421000001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/318873]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Ning |
| 作者单位 | 1.Guoke Ningbo Life Sci & Hlth Ind Res Inst, Ningbo, Zhejiang, Peoples R China 2.Hong Kong Univ Sci & Technol, Dept Elect & Comp Engn, Hong Kong, Peoples R China 3.Shenzhen Univ, Sch Biomed Engn, Med Sch, Shenzhen, Guangdong, Peoples R China 4.Hong Kong Baptist Univ, Dept Biol, State Key Lab Environm & Biol Anal, Hong Kong, Peoples R China 5.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan, Guangdong, Peoples R China 6.Guangdong Acad Sci, Inst Nanfan & Seed Ind, Guangzhou, Guangdong, Peoples R China 7.Hong Kong Univ Sci & Technol, Div Life Sci, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Kebin,Yang, Nan,Ren, Jia,et al. Cytosolic WPRa4 and Plastoskeletal PMI4 Proteins Mediate Touch Response in a Model Organism Arabidopsis[J]. MOLECULAR & CELLULAR PROTEOMICS,2025,24(7):22. |
| APA | Wu, Kebin.,Yang, Nan.,Ren, Jia.,Liu, Shichang.,Wang, Kai.,...&Li, Ning.(2025).Cytosolic WPRa4 and Plastoskeletal PMI4 Proteins Mediate Touch Response in a Model Organism Arabidopsis.MOLECULAR & CELLULAR PROTEOMICS,24(7),22. |
| MLA | Wu, Kebin,et al."Cytosolic WPRa4 and Plastoskeletal PMI4 Proteins Mediate Touch Response in a Model Organism Arabidopsis".MOLECULAR & CELLULAR PROTEOMICS 24.7(2025):22. |
入库方式： OAI收割
来源：上海药物研究所
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