ASB7 is a negative regulator of H3K9me3 homeostasis
文献类型：期刊论文
| 作者 | Zhou, Liwen5; Chen, Zhenxuan5; Zou, Yezi4; Zhang, Xia5; Wang, Zifeng5; Zhu, Hongwen3; Lin, Jiahui5; Huang, Ziyao5; Zheng, Lisi5; Chen, Jiali5 |
| 刊名 | SCIENCE
 |
| 出版日期 | 2025-07-17 |
| 卷号 | 389期号:6757页码:309-316 |
| ISSN号 | 0036-8075 |
| DOI | 10.1126/science.adq7408 |
| 通讯作者 | Zhou, Liwen(kangtb@sysucc.org.cn) ; Wu, Yuanzhong(wuyzh@sysucc.org.cn) ; Kang, Tiebang(zhoulw@sysucc.org.cn) |
| 英文摘要 | The maintenance of histone H3 lysine 9 trimethylation (H3K9me3) involves the recognition of preexisting modifications by heterochromatin protein 1 (HP1), which recruits the methyltransferase suppressor of variegation 3-9 homolog 1 (SUV39H1) to methylate the adjacent newly incorporated histones, establishing a positive feedback loop. However, how this positive feedback is restricted to maintain H3K9me3 homeostasis remains largely unknown. We performed an unbiased genome-scale CRISPR-Cas9 screen and identified CUL5ASB7 E3 ubiquitin ligase as a negative regulator of H3K9me3. ASB7 is recruited to heterochromatin by HP1 and promotes SUV39H1 degradation. During mitosis, cyclin-dependent kinase 1 (CDK1) phosphorylates ASB7, preventing its interaction with SUV39H1, leading to SUV39H1 stabilization and H3K9me3 restoration. Our findings reveal a dynamic circuit involving HP1, SUV39H1, and ASB7 that governs H3K9me3 homeostasis, ensuring faithful epigenetic inheritance and preventing excessive heterochromatin formation. |
| WOS关键词 | HETEROCHROMATIN PROTEIN-1 HP1 ; HISTONE H3 ; PHASE-SEPARATION ; BINDING-SITE ; LYSINE 9 ; CHROMATIN ; METHYLTRANSFERASE ; METHYLATION ; RECOGNITION ; COMPLEX |
| 资助项目 | National Key Research and Development Program of China[2021YFA1300601] ; National Natural Science Foundation of China[82341015] ; National Natural Science Foundation of China[82321003] ; National Natural Science Foundation of China[82273048] ; National Natural Science Foundation of China[82372591] ; National Natural Science Foundation of China[82030090] ; National Natural Science Foundation of China[82172939] ; Cancer Innovative Research Program of Sun Yat-sen University Cancer Center[CIRP-SYSUCC-0015] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001531570800021 |
| 出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/318876]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhou, Liwen; Wu, Yuanzhong; Kang, Tiebang |
| 作者单位 | 1.Peking Univ, Beijing Key Lab Tumor Syst Biol, Sch Basic Med Sci, Inst Syst Biomed,Hlth Sci Ctr,Dept Pathol, Beijing 100191, Peoples R China 2.South China Univ Technol, Guangzhou Peoples Hosp 1, Dept Hematol, Guangzhou, Peoples R China 3.Chinese Acad Sci, Dept Analyt Chem, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 4.Sun Yat sen Univ, Sch Med, Shenzhen Campus, Shenzhen 518107, Peoples R China 5.Sun Yat Sen Univ, Canc Ctr, State Key Lab Oncol South China, Guangdong Prov Clin Res Ctr Canc, Guangzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Liwen,Chen, Zhenxuan,Zou, Yezi,et al. ASB7 is a negative regulator of H3K9me3 homeostasis[J]. SCIENCE,2025,389(6757):309-316. |
| APA | Zhou, Liwen.,Chen, Zhenxuan.,Zou, Yezi.,Zhang, Xia.,Wang, Zifeng.,...&Kang, Tiebang.(2025).ASB7 is a negative regulator of H3K9me3 homeostasis.SCIENCE,389(6757),309-316. |
| MLA | Zhou, Liwen,et al."ASB7 is a negative regulator of H3K9me3 homeostasis".SCIENCE 389.6757(2025):309-316. |
入库方式： OAI收割
来源：上海药物研究所
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