Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques
文献类型：期刊论文
| 作者 | Li Lingnan; Yang Yi; Hou Xueling ; Gu Dongyu; Hang Ba; Abdulla Rahima; Wu Guirong; Xin Xuelei; Aisa Haji Akber
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| 刊名 | SEPARATION AND PURIFICATION TECHNOLOGY
 |
| 出版日期 | 2013 |
| 卷号 | 104期号:2页码:200-207 |
| 关键词 | Carthamus tinctorius L. Bioassay-guided separation Combination of chromatographic techniques High-speed counter-current chromatography Water-soluble antioxidants |
| ISSN号 | 1383-5866 |
| 通讯作者 | Aisa, HA |
| 中文摘要 | A combined chromatographic method using high-speed counter-current chromatography (HSCCC) and Sephadex LH-20 chromatography was established for bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L florets. The crude sample II obtained by an initial cleanup step on the AB-8 macroporous resin exhibited a potential ABTS radical cation scavenging activity with the SC50 value of 49.28 mu g/mL. The HSCCC separation was performed with a two-phase solvent system composed of n-butanol-0.1 mol/L HCl (1:1, v/v) at a flow rate of 1.2 mL/min. After a single run, 3 mg hydroxysafflor yellow A (HSYA) with 98% purity was separated as a major component from 20 mg of crude sample II. In order to increase the yield and investigate the minor components, the sample size of HSCCC separation was enlarged to 1 g. Then, HSCCC fractions were subjected to Sephadex LH-20 chromatography and eluted with distilled water at a flow rate of 1.5 mL/min to remove the acid and further separate the components. This separation yielded 184.0 mg of HSYA (1), 10.0 mg of 5,4'-dihydroxyflavone-3,6-di-O-beta-D-glucoside-7-O-beta-D-glucuronide (2), 3.0 mg of 3-O-caffeoylquinic acid (3), and 3.2 mg of 4-O-beta-D-glucosyl-trans-p-coumaric acid (4) from 1 g crude sample II of C tinctorius L. florets. The purity of the separated compounds were over 95% by HPLC analysis, and their chemical structures were confirmed by MS, H-1 NMR and C-13 NMR. Compounds 3 and 4 were found from this plant for the first time. Antioxidant activities assayed in vitro by ABTS radical cation scavenging showed that the SC50 values of the above four compounds were 44.39 +/- 1.62 mu g/mL, 78.13 +/- 1.00 mu g/mL, 21.85 +/- 1.96 mu g/mL, and 31.44 +/- 2.06 mu g/mL, respectively. (C) 2012 Elsevier B.V. All rights reserved. |
| 学科主题 | Engineering |
| 收录类别 | SCI |
| 资助信息 | Key Projects in the National Science & Technology Pillar Program 2011BAI05B05; High Tech Research and Development Program of Xinjiang 201010108; China National Funds for Distinguished Young Scientists 30925045 |
| 公开日期 | 2013-11-07 |
| 源URL | [http://ir.xjipc.cas.cn/handle/365002/2682]  |
| 专题 | 新疆理化技术研究所_省部共建新疆特有药用资源利用重点实验室 新疆理化技术研究所_资源化学研究室 |
| 作者单位 | Chinese Acad Sci, Xinjiang Tech Inst Phys & Chem, Key Lab Xinjiang Indigenous Med Plants Resource U, Urumqi 830011, Peoples R China;Xinjiang Med Univ, Sch Pharm, Urumqi 830054, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Lingnan,Yang Yi,Hou Xueling,et al. Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques[J]. SEPARATION AND PURIFICATION TECHNOLOGY,2013,104(2):200-207. |
| APA | Li Lingnan.,Yang Yi.,Hou Xueling.,Gu Dongyu.,Hang Ba.,...&Aisa Haji Akber.(2013).Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques.SEPARATION AND PURIFICATION TECHNOLOGY,104(2),200-207. |
| MLA | Li Lingnan,et al."Bioassay-guided separation and purification of water-soluble antioxidants from Carthamus tinctorius L. by combination of chromatographic techniques".SEPARATION AND PURIFICATION TECHNOLOGY 104.2(2013):200-207. |
入库方式： OAI收割
来源：新疆理化技术研究所
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