A sensitive ELISA made with recombinant antibodies for cynomolgus IL-8 in serum
文献类型：期刊论文
| 作者 | Yang, Leting2,3,4; Han, Xia1; Zhu, Xiao1; Wang, Yuhao1; Xu, Junjiu1; Chen, Yan3,4; Qin, Qiuping1; Gong, Likun1,2,3,4 |
| 刊名 | ANALYTICAL BIOCHEMISTRY
 |
| 出版日期 | 2026 |
| 卷号 | 708页码:9 |
| 关键词 | Cynomolgus monkeys Interleukin-8 Serum Quantitative assay ELISA |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2025.115991 |
| 通讯作者 | Qin, Qiuping(qpqin@cdser.simm.ac.cn) ; Gong, Likun(lkgong@cdser.simm.ac.cn) |
| 英文摘要 | Accurate measurement of interleukin-8 (IL-8) in non-human primates is crucial for research into inflammation and tumors; however, sensitive enzyme-linked immunosorbent assays (ELISAs) for non-clinical studies remain limited. We aimed to develop and validate a sensitive method for IL-8 quantification in cynomolgus monkey serum. Using two recombinant IL-8-specific antibodies and a surrogate matrix, a sandwich-type ELISA was developed, involving sequential incubations with samples, biotinylated antibody, and horseradish peroxidaselabeled Streptavidin (SA-HRP). The assay was validated against regulatory guidelines, achieving a lower limit of quantification of 4.69 pg/mL as well as good accuracy and precision. The assay was neither affected by serum matrix nor hemolysis, nor did it cross-react with other cytokines. Furthermore, the assay showed dilution linearity, no hook effect, and good recovery and parallelism. Finally, IL-8 in monkey serum exhibited sufficient stability for analysis. After validation, the assay was applied to 19 healthy cynomolgus monkey serum samples, and the results correlated well with those from a commercial assay. In conclusion, the developed ELISA is a sensitive, accurate, and reliable method for IL-8 quantification in cynomolgus monkey serum, suitable for nonclinical studies in cynomolgus monkeys. |
| WOS关键词 | REDUCED CLINICAL BENEFIT ; INTERLEUKIN-8 ; TEMPERATURE ; CYTOKINES |
| 资助项目 | Municipal Government of Zhongshan City ; Shanghai Institute of Materia Medica |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001604724700001 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/319714]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Qin, Qiuping; Gong, Likun |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Dept Clin Chem & Immunoassay, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 3.Guizhou Med Univ, Sch Pharmaceut Sci, Guiyang 561113, Peoples R China 4.Guizhou Med Univ, State Key Lab Discovery & Utilizat Funct Component, Guiyang 561113, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Leting,Han, Xia,Zhu, Xiao,et al. A sensitive ELISA made with recombinant antibodies for cynomolgus IL-8 in serum[J]. ANALYTICAL BIOCHEMISTRY,2026,708:9. |
| APA | Yang, Leting.,Han, Xia.,Zhu, Xiao.,Wang, Yuhao.,Xu, Junjiu.,...&Gong, Likun.(2026).A sensitive ELISA made with recombinant antibodies for cynomolgus IL-8 in serum.ANALYTICAL BIOCHEMISTRY,708,9. |
| MLA | Yang, Leting,et al."A sensitive ELISA made with recombinant antibodies for cynomolgus IL-8 in serum".ANALYTICAL BIOCHEMISTRY 708(2026):9. |
入库方式： OAI收割
来源：上海药物研究所
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