Boosting mRNA Therapeutics: pHLIP Enhances Endosomal Escape and Gene Expression in Lipid Nanoparticle
文献类型：期刊论文
| 作者 | Liu, Caixia8; Zhang, Kaixiang6,7; Ge, Ruijie7; Wang, Wenya5; Liu, Xueting6; Li, Xuan7; Hao, Pei2,3,4; Li, Honglin1,8 |
| 刊名 | ADVANCED HEALTHCARE MATERIALS
 |
| 出版日期 | 2025-10-24 |
| 页码 | 11 |
| 关键词 | endosomal escape lipid nanoparticles mRNA delivery mRNA vaccine pHLIP |
| ISSN号 | 2192-2640 |
| DOI | 10.1002/adhm.202502567 |
| 通讯作者 | Hao, Pei(phao@ips.ac.cn) ; Li, Honglin(hlli@ecust.edu.cn) |
| 英文摘要 | Messenger RNA (mRNA) has emerged as a transformative technology for combating viral pandemics and malignancies, with lipid nanoparticle (LNP)-based mRNA vaccines playing a pivotal role in the COVID-19 pandemic. However, current LNP face challenges such as low endosomal escape efficiency (<5%), leading to cargo waste and increased costs. To address this, pHLIP-incorporated LNP (mRNA@LNP-pHLIP), leveraging the pH-dependent membrane-insertion properties of pHLIP is developed to enhance endosomal escape. pHLIP undergoes a conformational change in acidic environments, enabling it to insert into endosomal membranes and promote the release of mRNA into the cytosol. In vitro studies demonstrate a three to fivefold increase in mRNA expression across multiple cell lines, while in vivo experiments show sustained and higher protein expression in mice. Applied to a monkeypox vaccine encoding A35R and M1R antigens, mRNA@LNP-pHLIP elicited stronger immune responses, highlighting its potential for next-generation mRNA therapeutics and vaccines. |
| WOS关键词 | ACCUMULATION ; TECHNOLOGY ; INSERTION ; DELIVERY |
| 资助项目 | Shanghai Post-doctoral Excellence Program |
| WOS研究方向 | Engineering ; Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001600887700001 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/319745]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Hao, Pei; Li, Honglin |
| 作者单位 | 1.East China Normal Univ, Innovat Ctr AI & Drug Discovery, Sch Pharm, Shanghai 200062, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Immun & Infect, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China 5.ChemPartner PharmaTech Co Ltd, Shanghai 200237, Peoples R China 6.East China Univ Sci Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 7.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Key Lab Synthet Biol, State Key Lab Plant Trait Design, Shanghai 200032, Peoples R China 8.Lingang Lab, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Caixia,Zhang, Kaixiang,Ge, Ruijie,et al. Boosting mRNA Therapeutics: pHLIP Enhances Endosomal Escape and Gene Expression in Lipid Nanoparticle[J]. ADVANCED HEALTHCARE MATERIALS,2025:11. |
| APA | Liu, Caixia.,Zhang, Kaixiang.,Ge, Ruijie.,Wang, Wenya.,Liu, Xueting.,...&Li, Honglin.(2025).Boosting mRNA Therapeutics: pHLIP Enhances Endosomal Escape and Gene Expression in Lipid Nanoparticle.ADVANCED HEALTHCARE MATERIALS,11. |
| MLA | Liu, Caixia,et al."Boosting mRNA Therapeutics: pHLIP Enhances Endosomal Escape and Gene Expression in Lipid Nanoparticle".ADVANCED HEALTHCARE MATERIALS (2025):11. |
入库方式： OAI收割
来源：上海药物研究所
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