Profiling the chemical differences of diterpenoid alkaloids in different processed products of Aconiti Lateralis Radix Praeparata by UHPLC-LTQ-Orbitrap mass spectrometry combined with untargeted metabolomics and mass spectrometry imaging
文献类型：期刊论文
| 作者 | Yu, Yang2; Yao, Changliang1; Zhang, Jianqing1; Huang, Yong1; Yao, Shuai1; Qu, Hua1; Zhang, Tong2; Guo, Dean1,2 |
| 刊名 | CHINESE JOURNAL OF NATURAL MEDICINES
 |
| 出版日期 | 2025-08-01 |
| 卷号 | 23期号:8页码:1009-1015 |
| 关键词 | Aconiti Lateralis Radix Praeparata Diterpenoid alkaloids Processing Untargeted metabolomics Mass spectrometry imaging |
| ISSN号 | 2095-6975 |
| DOI | 10.1016/S1875-5364(25)60936-8 |
| 通讯作者 | Zhang, Tong(zhangtongshutcm@hotmail.com) ; Guo, Dean(daguo@simm.ac.cn) |
| 英文摘要 | Aconiti Lateralis Radix Praeparata (Fuzi) represents a significant traditional Chinese medicine (TCM) that exhibits both notable pharmacological effects and toxicity. Various processing methods are implemented to reduce the toxicity of raw Fuzi by modifying its toxic and effective components, primarily diterpenoid alkaloids. To comprehensively analyze the chemical variations between different Fuzi products, ultra-high performance liquid chromatography-linear ion trap quadrupole Orbitrap mass spectrometry (UHPLC-LTQ-Orbitrap MS) was employed to systematically characterize Shengfuzi, Heishunpian and Baifupian. A total of 249 diterpenoid alkaloids present in Shengfuzi were identified, while only 111 and 61 in Heishunpian and Baifupian were detected respectively, indicating substantial differences among these products. An untargeted metabolomics approach combined with multivariate statistical analysis revealed 42 potential chemical markers. Through subsequent validation using 52 batches of commercial Heishunpian and Baifupian samples, 8 robust markers distinguishing these products were identified, including AC1-propanoic acid-3OH, HE-glucoside, HE-hydroxyvaleric acid-2OH, dihydrosphingosine, N-dodecoxycarbonylvaline and three unknown compounds. Additionally, the MS imaging (MSI) technique was utilized to visualize the spatial distribution of chemical constituents in raw Fuzi, revealing how different processing procedures affect the chemical variations between Heishunpian and Baifupian. The distribution patterns of different diterpenoid alkaloid subtypes partially explained the chemical differences among products. This research provides valuable insights into the material basis for future investigations of different Fuzi products. |
| WOS关键词 | ROOTS |
| 资助项目 | Qi-Huang Chief Scientist Program of the National Administration of Traditional Chinese Medicine (2020) ; National Key Research and Development Program of China[2022YFC3501705] ; Shanghai Sailing Program[23YF1447500] ; China Postdoctoral Science Foundation[2023M732335] |
| WOS研究方向 | Integrative & Complementary Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001593239900012 |
| 出版者 | CHINESE JOURNAL NATURAL MEDICINES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/320677]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhang, Tong; Guo, Dean |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Medica, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai 201203, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yu, Yang,Yao, Changliang,Zhang, Jianqing,et al. Profiling the chemical differences of diterpenoid alkaloids in different processed products of Aconiti Lateralis Radix Praeparata by UHPLC-LTQ-Orbitrap mass spectrometry combined with untargeted metabolomics and mass spectrometry imaging[J]. CHINESE JOURNAL OF NATURAL MEDICINES,2025,23(8):1009-1015. |
| APA | Yu, Yang.,Yao, Changliang.,Zhang, Jianqing.,Huang, Yong.,Yao, Shuai.,...&Guo, Dean.(2025).Profiling the chemical differences of diterpenoid alkaloids in different processed products of Aconiti Lateralis Radix Praeparata by UHPLC-LTQ-Orbitrap mass spectrometry combined with untargeted metabolomics and mass spectrometry imaging.CHINESE JOURNAL OF NATURAL MEDICINES,23(8),1009-1015. |
| MLA | Yu, Yang,et al."Profiling the chemical differences of diterpenoid alkaloids in different processed products of Aconiti Lateralis Radix Praeparata by UHPLC-LTQ-Orbitrap mass spectrometry combined with untargeted metabolomics and mass spectrometry imaging".CHINESE JOURNAL OF NATURAL MEDICINES 23.8(2025):1009-1015. |
入库方式： OAI收割
来源：上海药物研究所
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