Hypoglycemic potential of American ginseng saponins: inhibition of α-amylase and improvement of insulin resistance
文献类型：期刊论文
| 作者 | Liang, Liwen3,4; Guo, Yunlong3; Yao, Xu2; Cai, Guangzhi3; Liu, Xiaokang1,3; Gong, Jiyu3 |
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2025-12-15 |
| 卷号 | 1267页码:10 |
| 关键词 | American ginseng total saponins alpha-Amylase inhibition Insulin resistance Molecular docking Diabetes mellitus |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2025.124813 |
| 通讯作者 | Liu, Xiaokang(liuxiaokang0583@zidd.ac.cn) ; Gong, Jiyu(gjy0431@126.com) |
| 英文摘要 | Diabetes Mellitus (DM) represents a significant global health challenge, necessitating the development of novel therapeutic agents with reduced adverse effects for effective management. This study investigated the hypoglycemic potential of total saponins derived from American ginseng (TSAG), focusing on its inhibitory activity against alpha-amylase and its modulatory effects on insulin resistance in HepG2 cells. Results from the alpha-amylase inhibition assay demonstrated that TSAG elicited significant, concentration-dependent inhibition. Enzyme kinetic analysis revealed that TSAG functions as a reversible, mixed-type inhibitor of alpha-amylase. In insulin-resistant HepG2 cells, TSAG significantly enhanced glucose consumption and promoted glycogen synthesis, indicating its capacity to ameliorate insulin sensitivity. Ultra-high-performance liquid chromatography coupled with quadrupole orbitrap mass spectrometry (UHPLC-Q-Orbitrap/MS) analysis identified 40 ginsenoside constituents within TSAG. Molecular docking studies demonstrated high-affinity binding interactions between these key ginsenosides and alpha-amylase, corroborating their contribution to the observed inhibitory effects. Collectively, these findings highlight the therapeutic potential of American ginseng saponins in diabetes management, particularly in improving postprandial glycemic control and enhancing insulin sensitivity. |
| WOS关键词 | PANAX-QUINQUEFOLIUM ; RG3 |
| 资助项目 | Natural Science Fund Project of Jilin Province, China[20210204078YY] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001596623900001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/320687]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Xiaokang; Gong, Jiyu |
| 作者单位 | 1.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 2.Leiyun Shang Pharmaceut Co Ltd, Anhui 236000, Peoples R China 3.Changchun Univ Chinese Med, Sch Pharmaceut Sci, Changchun 130117, Peoples R China 4.Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Shenyang 110016, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Liwen,Guo, Yunlong,Yao, Xu,et al. Hypoglycemic potential of American ginseng saponins: inhibition of α-amylase and improvement of insulin resistance[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2025,1267:10. |
| APA | Liang, Liwen,Guo, Yunlong,Yao, Xu,Cai, Guangzhi,Liu, Xiaokang,&Gong, Jiyu.(2025).Hypoglycemic potential of American ginseng saponins: inhibition of α-amylase and improvement of insulin resistance.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,1267,10. |
| MLA | Liang, Liwen,et al."Hypoglycemic potential of American ginseng saponins: inhibition of α-amylase and improvement of insulin resistance".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1267(2025):10. |
入库方式： OAI收割
来源：上海药物研究所
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