Alleviation of dry eye disease with lyophilized extracellular vesicles
文献类型：期刊论文
| 作者 | Ren, Xiaohong5; Lin, Xueyuan4; Li, Feng1,5; Zheng, Shiyu3; Wen, Yuewen3; Zhang, Guoqing5; Yang, Rui2; Wu, Li5; Zhang, Jiwen1,3,4,5 |
| 刊名 | JOURNAL OF CONTROLLED RELEASE
 |
| 出版日期 | 2025-09-10 |
| 卷号 | 385页码:11 |
| 关键词 | Dry eye disease Extracellular vesicles Lyophilization Ectoine Stability enhancement Therapeutic efficacy |
| ISSN号 | 0168-3659 |
| DOI | 10.1016/j.jconrel.2025.114044 |
| 通讯作者 | Zhang, Jiwen(jwzhang@simm.ac.cn) |
| 英文摘要 | Dry eye disease (DED), a prevalent ocular condition affecting millions globally, necessitates the development of innovative therapeutic strategies for long-term management. Although extracellular vesicles (EVs) have shown therapeutic potential for DED, their clinical translation has been substantially limited by stability issues during storage. In this study, a novel lyophilization strategy incorporating ectoine-a natural osmolyte with superior biostabilization capabilities-was developed to address these challenges. For the first time, an ectoine-enhanced lyophilization protocol was established to preserve the key functional properties of milk-derived EVs (mEVs) during prolonged storage at 4 degrees C. Comprehensive characterization through hydrodynamic analysis, structural evaluation, beta-galactosidase activity assessment, and functional validation demonstrated that mEVs lyophilized with 0.5 %-4 % ectoine maintained critical biological properties for over two months, representing a significant improvement compared to conventional preservation methods. The lyophilized mEVs were shown to effectively protect human corneal epithelial cells (HCECs) against hyperosmolarity-induced damage. Notably, in a benzalkonium chloride (BAC)-induced rabbit model of DED, the optimized formulation exhibited enhanced therapeutic performance, as evidenced by significant improvements in clinical parameters and ocular surface health. These findings established ectoine-based lyophilization as a transformative preservation methodology for EVbased therapeutics, providing a robust solution to persistent stability challenges in ocular drug delivery systems and facilitating the clinical translation of EV-based treatments for DED and related ocular surface disorders. |
| WOS关键词 | EXOSOMES ; MODEL |
| 资助项目 | National Natural Science Foundation of China[82273863] ; Shanghai Institute of Material Medica, Chinese Academy of Sciences |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001539839700001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321120]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhang, Jiwen |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Natl Inst Food & Drug Control, Beijing 100050, Peoples R China 3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China 4.Shenyang Pharmaceut Univ, Shenyang 110016, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Xiaohong,Lin, Xueyuan,Li, Feng,et al. Alleviation of dry eye disease with lyophilized extracellular vesicles[J]. JOURNAL OF CONTROLLED RELEASE,2025,385:11. |
| APA | Ren, Xiaohong.,Lin, Xueyuan.,Li, Feng.,Zheng, Shiyu.,Wen, Yuewen.,...&Zhang, Jiwen.(2025).Alleviation of dry eye disease with lyophilized extracellular vesicles.JOURNAL OF CONTROLLED RELEASE,385,11. |
| MLA | Ren, Xiaohong,et al."Alleviation of dry eye disease with lyophilized extracellular vesicles".JOURNAL OF CONTROLLED RELEASE 385(2025):11. |
入库方式： OAI收割
来源：上海药物研究所
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