Structural characterization of two α-glucans with different molecular weights from Flemingia philippinensis and their immune-enhancing activities on cyclophosphamide-treated mice through regulation of gut microbiota
文献类型：期刊论文
| 作者 | Ma, Xiaoxu2,3; Wang, Guanying2,3; Yang, Le2,3; Liu, Haichao2,3; Zou, Yanqi2,3; Zhang, Daidi2; An, Yaling2; Liu, Xiaokang2; Li, Zhenwei2; Guo, De-an1,2,3 |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2025-09-01 |
| 卷号 | 321页码:13 |
| 关键词 | Glucan Flemingia philippinensis Immune-enhancing Gut microbiota Structure-property relationships Molecular weight |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2025.146555 |
| 通讯作者 | Li, Zhenwei(lizhenwei@zidd.ac.cn) ; Guo, De-an(daguo@simm.ac.cn) |
| 英文摘要 | The roots of Flemingia philippinensis have long been used as a folk medicine and food ingredient in China. However, polysaccharides from F. philippinensis remain largely unexplored. Therefore, the objective of this study was to elucidate the polysaccharide material basis of F. philippinensis and evaluate its immune-enhancing potential. Using an optimized hot water extraction method, two homogeneous polysaccharides, FPP1 and FPP2, were isolated from F. philippinensis for the first time. Comprehensive structural characterization revealed FPP1 and FPP2 as alpha-1,4-linked glucans with comparable physicochemical properties and primary structures. The main differences laid in their molecular weight, of which were 1.60 x 105 and 9.78 x 104 Da, respectively. Besides, the solution conformation of FPP2 was more branched than FPP1. Critically, treatment with FPP1 and FPP2 alleviated the cyclophosphamide (CTX)-induced immunosuppression in mice, and ameliorated the CTX-induced gut microbiota dysbiosis. Notably, FPP2 demonstrated superior immune-enhancing efficacy and more pronounced improvements in gut microbiota, particularly in modulating the Bacteroidota and Akkermansia; whereas FPP1 exhibited a stronger restorative effect on the Firmicutes. Furthermore, the structure-property relationships of FPP1 and FPP2 were explored to provide new insights for future researches on glucans. Collectively, these findings highlighted F. philippinensis-derived glucans, particularly FPP2, as promising immunomodulatory agents through regulation of gut microbiota. |
| WOS关键词 | POLYSACCHARIDES |
| 资助项目 | Guangdong Basic and Applied Basic Research Foundation[2023A1515111180] ; National Natural Science Foundation of China[32141005] ; Key Program of National Natural Science Foundation of China[82130111] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001548177100007 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321255]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Zhenwei; Guo, De-an |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Haike Rd 501, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Heqing Rd 10, Zhongshan 528400, Peoples R China 3.Guangzhou Univ Chinese Med, Guangzhou 510006, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Xiaoxu,Wang, Guanying,Yang, Le,et al. Structural characterization of two α-glucans with different molecular weights from Flemingia philippinensis and their immune-enhancing activities on cyclophosphamide-treated mice through regulation of gut microbiota[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2025,321:13. |
| APA | Ma, Xiaoxu.,Wang, Guanying.,Yang, Le.,Liu, Haichao.,Zou, Yanqi.,...&Guo, De-an.(2025).Structural characterization of two α-glucans with different molecular weights from Flemingia philippinensis and their immune-enhancing activities on cyclophosphamide-treated mice through regulation of gut microbiota.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,321,13. |
| MLA | Ma, Xiaoxu,et al."Structural characterization of two α-glucans with different molecular weights from Flemingia philippinensis and their immune-enhancing activities on cyclophosphamide-treated mice through regulation of gut microbiota".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 321(2025):13. |
入库方式： OAI收割
来源：上海药物研究所
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