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A novel method to quantify Dendrobium officinale polysaccharides in biological samples via 99mTc-labelling and radioHPGPC analysis

文献类型：期刊论文

作者Dou, Pengfei9; Wang, Jinglin6,7,8; Shu, Yamin5; Cheng, Fang6,7,8; Nie, Gang4; Luo, Li6,7,8; Wu, Zhijing6,7,8; Zheng, Ziming6,7,8; Gai, Yongkang3; Liu, Junxi6,7,8
刊名CARBOHYDRATE POLYMERS
出版日期2025-11-01
卷号367页码:13
关键词Polysaccharide labelling Pharmacokinetic study Radioisotopic labelling Radio-HPLC
ISSN号0144-8617
DOI10.1016/j.carbpol.2025.123935
通讯作者Zhang, Yu(zhangwkp@163.com) ; Lan, Xiaoli(LXL730724@hotmail.com) ; Ding, Kan(dingkan@simm.ac.cn) ; Wang, Kaiping(wkpzcq@163.com)
英文摘要Natural polysaccharides are attracting increasing attention due to their excellent pharmacological properties and low toxicity. However, the clinical translation of these biomacromolecules has been hindered by a lack of pharmacokinetic studies. Polysaccharide labelling presents a promising method for performing pharmacokinetic investigations on polysaccharides. The combination of radioisotopic labelling and high-performance gel permeation chromatography (HPGPC) has not yet been applied to the detection of polysaccharides in biological samples. Here, Dendrobium officinale polysaccharides (DOP) were selected as model polysaccharides. DOP and radiolabelling of DOP ((TC)-T-99m-DTPA-DOP) were prepared and characterized. Then, HPGPC with a radio-HPLC flow detector (radioHPGPC) was used to analyse (TC)-T-99m-DTPA-DOP in biological samples for the first time. The detection method was demonstrated to exhibit good linearity (R-2 > 0.997), sensitivity (LLOD <100 nCi, and 62.99 ng/mL for the Conc of DOP), specificity, and recovery rate (>90 %). The blood concentration and distribution in major organs after oral and intravenous administration were studied. The results showed that the bioavailability of DOP was 2.68 %. This method is superior to gamma counting or liquid scintillation counting because radioHPGPC could not only eliminate the impact of free Tc-99m and quantity the DOP tracer but also qualitatively analyse the metabolism during absorption and distribution. This study provides detailed pharmacokinetic profiles of DOP and a practical method for labelling and pharmacokinetic studies of other polysaccharides with high molecular weight.
WOS关键词TISSUE DISTRIBUTION ; PHARMACOKINETICS ; MICROANALYSIS
资助项目National Natural Science Foundation of China[82074111] ; National Natural Science Foundation of China[U23A20536] ; Interdisciplinary Research Program of HUST[2023JCYJ038]
WOS研究方向Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001555385700001
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.183/handle/2S10ELR8/321315]  
专题中国科学院上海药物研究所
通讯作者Zhang, Yu; Lan, Xiaoli; Ding, Kan; Wang, Kaiping
作者单位1.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China
3.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Nucl Med, 1277 Jiefang Ave, Wuhan 430022, Peoples R China
4.Huazhong Univ Sci & Technol, Wuhan Maternal & Child Healthcare Hosp, Wuhan Childrens Hosp, Tongji Med Coll,Dept Pharm, Wuhan 430016, Peoples R China
5.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Dept Pharm, Wuhan 430030, Peoples R China
6.Hubei Prov Clin Res Ctr Precis Med Crit Illness, Wuhan 430030, Peoples R China
7.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Hubei Key Lab Nat Act Polysaccharides, Wuhan 430022, Hubei, Peoples R China
8.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Dept Pharm, Wuhan 430030, Peoples R China
9.Huazhong Univ Sci & Technol, Tongji Med Coll Pharm, Wuhan 430030, Peoples R China
推荐引用方式
GB/T 7714		 Dou, Pengfei,Wang, Jinglin,Shu, Yamin,et al. A novel method to quantify Dendrobium officinale polysaccharides in biological samples via 99mTc-labelling and radioHPGPC analysis[J]. CARBOHYDRATE POLYMERS,2025,367:13.
APA Dou, Pengfei.,Wang, Jinglin.,Shu, Yamin.,Cheng, Fang.,Nie, Gang.,...&Wang, Kaiping.(2025).A novel method to quantify Dendrobium officinale polysaccharides in biological samples via 99mTc-labelling and radioHPGPC analysis.CARBOHYDRATE POLYMERS,367,13.
MLA Dou, Pengfei,et al."A novel method to quantify Dendrobium officinale polysaccharides in biological samples via 99mTc-labelling and radioHPGPC analysis".CARBOHYDRATE POLYMERS 367(2025):13.

入库方式： OAI收割

来源：上海药物研究所

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