A network pharmacology approach and experimental validation to investigate the protection mechanism of Ganoderma lucidum (Leyss. ex Fr.) Karst spore for acute kidney injury through induction of apoptosis via p53/ Caspase 3 signaling pathway
文献类型：期刊论文
| 作者 | Zhou, Yue3; Zhang, Baowen1,2,3; Wang, Xiaodan3; Liu, Xiaoqin3; Zhao, Jinna3; Wang, Yanli3; Gan, Chunli3 |
| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2025-07-24 |
| 卷号 | 351页码:12 |
| 关键词 | Acute kidney injury Ganoderma lucidum (leyss. ex Fr.) Karst spore Molecular docking p53 |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2025.120075 |
| 通讯作者 | Gan, Chunli(chunligan@126.com) |
| 英文摘要 | Ethnopharmacological relevance: According to the Sheng Nong's herbal classic, one of the components Ganoderma lucidum (Leyss. ex Fr.) "benefits waterways and kidney qi". The reproductive cells of Ganoderma lucidum (Leyss. ex Fr.) Karst spore (GLS) has richer active substances, and the protective mechanism against acute kidney injury (AKI) needs to be clarified. Purpose: This study dedicates to evaluate the effect of GLS on AKI, explores activation of the p53 signaling pathway, and investigates potential apoptosis-regulating mechanism. Methods: Gentamicin (GM) was used to establish an AKI rat model by intraperitoneal injection followed by the GLS intervention. The therapeutic efficacy of the GLS was evaluated based on function indices in rats. Network pharmacology was used to identify key proteins for the GLS intervention in AKI rats and bioinformatics analysis were performed. The initiation of the p53 signaling pathway was verified by molecular biology and surface enhanced raman spectroscopy (SERS). Results: UPLC-Q-Tof MS was carried to characterize the triterpene saponin analogs extracted from the GLS. The molecular docking results of the protein Caspase 3 showed that Autodock Binding energy was 8.86, and PyRx Binding Affinity was 9.6. Successfully established an AKI rat model. Compared with the GM group, the serum renal function markers in GLS dose groups were decreased; the degree of renal tissue lesions, renal tubular injury and inflammatory cell infiltration in GLS dose groups were significantly reduced, the expression of p53, Cytochrome C, BAX, Caspase-3 and Cleaved Caspase-3 proteins in the renal tissues of GLS dose groups were downregulated, and the expression of BcL-2 protein was up-regulated. TUNEL staining and SERS also showed that GLS significantly inhibited the hyperapoptosis of kidney tissue caused by GM. This is the first study applying the GLS to treat GM-induced AKI. Conclusion: This study was innovatively proposed that the GLS exerts a protective effect against AKI by inhibiting p53 overexpression. In addition, SERS was firstly applied to the spectroscopic observation of renal apoptotic tissues, and the results indicated that the GLS had a role in down-regulating excessive apoptosis in the injured kidney. |
| WOS关键词 | RAMAN-SPECTROSCOPY ; ACID |
| 资助项目 | Heilongjiang Provincial Postdoctoral Science Foundation[21042220041] ; Fundamental Research Funds for the Provincial Universities[JFQN202408] ; Key Commissioned Project for Undergraduate Education Reform in Heilongjiang Province, China[SJGZ20220079] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001566287800001 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321435]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Gan, Chunli |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China 3.Harbin Med Univ, Coll Pharm, Dept Pharmaceut Anal & Analyt Chem, 157 Baojian Rd, Harbin 150081, Heilongjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yue,Zhang, Baowen,Wang, Xiaodan,et al. A network pharmacology approach and experimental validation to investigate the protection mechanism of Ganoderma lucidum (Leyss. ex Fr.) Karst spore for acute kidney injury through induction of apoptosis via p53/ Caspase 3 signaling pathway[J]. JOURNAL OF ETHNOPHARMACOLOGY,2025,351:12. |
| APA | Zhou, Yue.,Zhang, Baowen.,Wang, Xiaodan.,Liu, Xiaoqin.,Zhao, Jinna.,...&Gan, Chunli.(2025).A network pharmacology approach and experimental validation to investigate the protection mechanism of Ganoderma lucidum (Leyss. ex Fr.) Karst spore for acute kidney injury through induction of apoptosis via p53/ Caspase 3 signaling pathway.JOURNAL OF ETHNOPHARMACOLOGY,351,12. |
| MLA | Zhou, Yue,et al."A network pharmacology approach and experimental validation to investigate the protection mechanism of Ganoderma lucidum (Leyss. ex Fr.) Karst spore for acute kidney injury through induction of apoptosis via p53/ Caspase 3 signaling pathway".JOURNAL OF ETHNOPHARMACOLOGY 351(2025):12. |
入库方式： OAI收割
来源：上海药物研究所
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