CDC42 supports HBV entry by NTCP translocation to the plasma membrane and macropinocytosis
文献类型：期刊论文
| 作者 | Cui, Shuzhi5,6; Gao, Wei3,4,5; Chen, Yuxin2; Xu, Yi6; Li, Zhifang5; Wei, Yu1,3; Jiu, Yaming3,4,5 |
| 刊名 | EMBO REPORTS
 |
| 出版日期 | 2025-09-15 |
| 页码 | 31 |
| 关键词 | CDC42 Hepatitis B Virus Macropinocytosis Sodium Taurocholate Cotransporting Polypeptide Virus Entry |
| ISSN号 | 1469-221X |
| DOI | 10.1038/s44319-025-00581-8 |
| 通讯作者 | Wei, Yu(yu.wei@pasteur.fr) ; Jiu, Yaming(ymjiu@siii.cas.cn) |
| 英文摘要 | CDC42 is a member of Rho GTPase family that regulates various biological processes and its activity can be hijacked by invading pathogens. Here, we discovered that the level of active CDC42 in hepatocytes positively correlates with the entry capacity of hepatitis B virus (HBV). Mechanistically, CDC42 activation effectively promotes the transport of the viral receptor sodium taurocholate co-transporting polypeptide (NTCP) to the plasma membrane via Rab11 dependent recycling endosomal pathway. NTCP interacts with Rab11 and activation of CDC42 signaling reinforces the interaction between NTCP and Rab11. We further show that clathrin mediated endocytosis (CME), the known HBV entry pathway, is independent of CDC42 activity. Intriguingly, we reveal that CDC42 dependent macropinocytosis is a route for HBV entry, which is equally essential for viral infection as CME. Together, our findings uncover new mechanisms for HBV entry that involve unrecognized functions of CDC42 and suggest that Rho GTPase signaling might represent a potential target for antiviral therapy. |
| WOS关键词 | HEPATITIS-B-VIRUS ; CO-TRANSPORTING POLYPEPTIDE ; CELL ENTRY ; BINDING ; PROTEINS ; RECEPTOR ; COTRANSPORT ; HEPATOCYTES ; EXPRESSION ; INHIBITOR |
| 资助项目 | MOST | National Natural Science Foundation of China (NSFC)[32222022] ; MOST | National Natural Science Foundation of China (NSFC)[92354301] ; MOST | National Natural Science Foundation of China (NSFC)[92054104] ; National Natural Science Foundation of China[GZNL2023A03004] ; R&D Program of Guangzhou National Laboratory[23ZR1470900] ; Natural Science Foundation of Shanghai[2022YFC2303502] ; Key Research and Development Program, Ministry of Science and Technology of China |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001571207600001 |
| 出版者 | SPRINGERNATURE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321474]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wei, Yu; Jiu, Yaming |
| 作者单位 | 1.Univ Paris Cite, Inst Pasteur, 28 Rue Dr Roux, F-75015 Paris, France 2.Nanjing Univ, Nanjing Drum Tower Hosp, Dept Lab Med, Med Sch,Affiliated Hosp, Nanjing 210008, Peoples R China 3.Univ Chinese Acad Sci, Yuquan Rd 19 A),Shijingshan Dist, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China 5.Chinese Acad Sci, Shanghai Inst Immun & Infect, Unit Cell Biol & Imaging Study Pathogen Host Inter, Key Lab Mol Virol & Immunol, Shanghai 200031, Peoples R China 6.Guangzhou Med Univ, Guangzhou Inst Pediat, Guangzhou Women & Childrens Med Ctr, Guangzhou 510623, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Shuzhi,Gao, Wei,Chen, Yuxin,et al. CDC42 supports HBV entry by NTCP translocation to the plasma membrane and macropinocytosis[J]. EMBO REPORTS,2025:31. |
| APA | Cui, Shuzhi.,Gao, Wei.,Chen, Yuxin.,Xu, Yi.,Li, Zhifang.,...&Jiu, Yaming.(2025).CDC42 supports HBV entry by NTCP translocation to the plasma membrane and macropinocytosis.EMBO REPORTS,31. |
| MLA | Cui, Shuzhi,et al."CDC42 supports HBV entry by NTCP translocation to the plasma membrane and macropinocytosis".EMBO REPORTS (2025):31. |
入库方式： OAI收割
来源：上海药物研究所
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