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A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges

文献类型：期刊论文

作者Lin, Jia-Ling1,2,3; Wu, Wen-Kai1; Nie, Gui-Bin1; Li, Jian-Xu1,4; Fang, Xin5; Sheng, Yin-Guo1; Wang, Meng-Meng1,2; Zheng, Qi-Yue1; Guo, Xiao-Xiang1; Huang, Jia-Fa1
刊名NATURE COMMUNICATIONS
出版日期2025-10-20
卷号16期号:1页码:19
DOI10.1038/s41467-025-64323-z
英文摘要Plants have evolved an extensive repertoire of specialized metabolites to adapt to complex environmental changes. Here, we identify two paralogous dirigent proteins (DPs) in cotton that serve as gatekeepers of extracellular terpenoid phytoalexin production in green organs, directing the transition of hemigossypol away from gossypol synthesis toward a hydroxylation pathway that leads to the biosynthesis of highly toxic hemigossypolone and heliocides. Under oxidative conditions, these proteins function synergistically with aldo-keto reductases to catalyze the hydroxylation of hemigossypol, followed by spontaneous oxidation that yields hemigossypolone, revealing a noncanonical role for aldo-keto reductases in extracellular terpenoid metabolism. Notably, mutants lacking these dirigent proteins produce gossypol but are devoid of hemigossypolone and heliocides in green organs exhibit heightened susceptibility to multiple biotic stresses, underscoring the enhanced protective role of these metabolites. This study describes a DPs-mediated mechanism of extracellular hydroxylation and highlights the potential ecological advantages of redirecting specialized metabolism extracellularly for enhanced defense against varying types of pathogens and herbivores.
WOS关键词NATURAL-PRODUCTS ; CONIFER DEFENSE ; GENE DISCOVERY ; EXPRESSION ; GOSSYPOL ; COTTON ; PHYLOGENY ; SYNTHASE ; RESISTANCE ; EVOLUTION
资助项目National Natural Science Foundation of China (National Science Foundation of China)[2022YFD1400800] ; National Key R&D Program of China[32388201] ; National Key R&D Program of China[32300330] ; National Key R&D Program of China[32422007] ; National Natural Science Foundation of China[CASSHB-QNPD-2023-015] ; Talent Plan of Shanghai Branch, Chinese Academy of Sciences[2022273] ; Foundation of Youth Innovation Promotion Association of the Chinese Academy of Sciences[202305AT350001] ; Yunnan Revitalization Talent Support Program Top Team Project[tsqn202312303] ; Taishan Scholars Program[24QA2710300] ; Taishan Scholars Program[23QA1410100] ; Shanghai Rising-Star Project[No.T2024204] ; Shanghai Agricultural Science and Technology Innovation Program[2022YFC2804100] ; National Key Research and Development Program of China[SYS202205] ; Shandong Laboratory Program[23PJ1415300] ; Shanghai Pujiang Program[GMLM2023] ; Winall Hi-tech Seed Co., Ltd.
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:001597726000014
出版者NATURE PORTFOLIO
源URL[http://119.78.100.183/handle/2S10ELR8/320704]  
专题国家级研究中心_原创新药研究全国重点实验室
通讯作者Huang, Jin-Quan
作者单位1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Plant Physiol & Ecol, CAS Ctr Excellence Mol Plant Sci,State Key Lab Pla, Shanghai, Peoples R China
2.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China
3.MIT, Synthet Biol Ctr, Dept Biol Engn, Cambridge, MA USA
4.Chinese Acad Sci, Shanghai Chenshan Plant Sci Res Ctr, Shanghai Key Lab Plant Funct Genom & Resources, Shanghai Chenshan Bot Garden, Shanghai, Peoples R China
5.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources Wes, Kunming, Yunnan, Peoples R China
6.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Organ Chem, Ctr Excellence Mol Synth,CAS Key Lab Energy Regula, Shanghai, Peoples R China
7.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai, Shandong, Peoples R China
8.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
9.Shenzhen Univ, Coll Life Sci & Oceanog, Guangdong Prov Key Lab Plant Epigenet, Shenzhen, Peoples R China
10.Xinjiang Jinfengyuan Seeds Co Ltd, Aksu, Xinjiang, Peoples R China
推荐引用方式
GB/T 7714		 Lin, Jia-Ling,Wu, Wen-Kai,Nie, Gui-Bin,et al. A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges[J]. NATURE COMMUNICATIONS,2025,16(1):19.
APA Lin, Jia-Ling.,Wu, Wen-Kai.,Nie, Gui-Bin.,Li, Jian-Xu.,Fang, Xin.,...&Huang, Jin-Quan.(2025).A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges.NATURE COMMUNICATIONS,16(1),19.
MLA Lin, Jia-Ling,et al."A dirigent protein redirects extracellular terpenoid metabolism for defense against biotic challenges".NATURE COMMUNICATIONS 16.1(2025):19.

入库方式： OAI收割

来源：上海药物研究所

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