A Metabolomics Exploration of Young Lotus Seeds Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging
文献类型：期刊论文
| 作者 | Chen, Ying1,3; Xu, Xiaomeng2; Tang, Chunping3 |
| 刊名 | MOLECULES
 |
| 出版日期 | 2025-08-01 |
| 卷号 | 30期号:15页码:15 |
| 关键词 | louts seed plumule MALDI-MSI secondary metabolites spatial distribution |
| DOI | 10.3390/molecules30153242 |
| 英文摘要 | Lotus (Nelumbo nucifera Gaertn.) is a quintessential medicinal and edible plant, exhibiting marked differences in therapeutic effects among its various parts. The lotus seed constitutes a key component of this plant. Notably, the entire seed and the plumule display distinct medicinal properties. To investigate the "homologous plants with different effects" phenomenon in traditional Chinese medicine, this study established a Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging (MALDI-MSI) method. This study employed immature lotus seeds as the experimental material, diverging from the mature seeds conventionally used. Conductive double-sided tape was employed for sample preparation, and complete longitudinal sections of the seeds were obtained, followed by MALDI-MSI analysis to identify and visualize the spatial distribution of characteristic secondary metabolites within the entire seeds. The results unveiled the diversity of metabolites in lotus seeds and their differential distribution across tissues, with pronounced distinctions in the plumule. A total of 152 metabolites spanning 13 categories were identified in lotus seeds, with 134, 89, 51, and 98 metabolites discerned in the pericarp, seed coat, cotyledon, and plumule, respectively. Strikingly, young lotus seeds were devoid of liensinine/isoliensinine and neferine, the dominant alkaloids of mature lotus seed plumule, revealing an early-stage alkaloid profile that sharply contrasts with the well-documented abundance found in mature seeds and has rarely been reported. We further propose a biosynthetic pathway to explain the presence of the detected benzylisoquinoline and the absence of the undetected bisbenzylisoquinoline alkaloids in this study. These findings present the first comprehensive metabolic atlas of immature lotus seeds, systematically exposing the pronounced chemical divergence from their mature counterparts, and thus lays a metabolomic foundation for dissecting the spatiotemporal mechanisms underlying the nutritional and medicinal value of lotus seeds. |
| WOS关键词 | NELUMBO-NUCIFERA ; ANTIOXIDANT ; FLAVONOIDS ; FERULOYLTYRAMINE ; ALKALOIDS ; TISSUES ; LEAVES ; ACID |
| 资助项目 | Key-Area Research and Development Program of Guangdong Province[2020B0303070002] ; Key-Area Research and Development Program of Guangdong Province ; National Key R&D Program of China[2024YFE0117200] ; International Governmental S&T Innovation Cooperation Project ; Ministry of Science and Technology of China |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001549450100001 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321267]  |
| 专题 | 国家级研究中心_原创新药研究全国重点实验室 |
| 通讯作者 | Tang, Chunping |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Henan Univ, Sch Pharm, Kaifeng 475004, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Ying,Xu, Xiaomeng,Tang, Chunping. A Metabolomics Exploration of Young Lotus Seeds Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging[J]. MOLECULES,2025,30(15):15. |
| APA | Chen, Ying,Xu, Xiaomeng,&Tang, Chunping.(2025).A Metabolomics Exploration of Young Lotus Seeds Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging.MOLECULES,30(15),15. |
| MLA | Chen, Ying,et al."A Metabolomics Exploration of Young Lotus Seeds Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging".MOLECULES 30.15(2025):15. |
入库方式： OAI收割
来源：上海药物研究所
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