Direct Identification of O-Glycopeptides by Low-Temperature Assisted Nanopore Technique
文献类型：期刊论文
| 作者 | Wang, Jia-Hong1; Ma, Wenjing2; Hu, Zheng-Li1; Gao, Zhaobing3; Long, Yi-Tao1; Li, Tiehai2; Ying, Yi-Lun1,4 |
| 刊名 | RESEARCH
 |
| 出版日期 | 2025-09-04 |
| 卷号 | 8页码:10 |
| ISSN号 | 2096-5168 |
| DOI | 10.34133/research.0850 |
| 英文摘要 | O-glycopeptides are highly expressed in various human cancers and play a key role in cancer progression and metastasis, making them promising biomarkers for early diagnostics. However, the inherent complexity and heterogeneity of glycans pose a major challenge for the simultaneous and precise analysis of multiple glycopeptides. Here, we developed a low-temperature nanopore technique capable of simultaneously discriminating 4 truncated O-glycopeptides with varied glycoforms. This method enables the direct identification and relative quantification of O-glycopeptides from a mixture, achieving a discrimination accuracy of 92.9%. This general strategy holds promise for the label-free analysis of glycopeptide biomarkers, with potential applications in cancer diagnostics. |
| WOS关键词 | OXIDATIVE RELEASE ; GLYCANS ; GLYCOSYLATION ; AEROLYSIN ; PEPTIDES ; PROTEINS |
| 资助项目 | National Key R&D Program of China[2022YFA1304604] ; Shanghai Municipal Science and Technology Major Project ; National Natural Science Foundation of China[22334006] ; National Natural Science Foundation of China[22077130] ; National Natural Science Foundation of China[22377134] ; Excellent Research Program of Nanjing University[ZYJH004] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001564497700001 |
| 出版者 | AMER ASSOC ADVANCEMENT SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321405]  |
| 专题 | 国家级研究中心_原创新药研究全国重点实验室 |
| 通讯作者 | Gao, Zhaobing; Li, Tiehai; Ying, Yi-Lun |
| 作者单位 | 1.Nanjing Univ, Mol Sensing & Imaging Ctr, Sch Chem & Chem Engn, Nanjing 210023, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Nanjing Univ, Chem & Biomed Innovat Ctr, Nanjing 210023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jia-Hong,Ma, Wenjing,Hu, Zheng-Li,et al. Direct Identification of O-Glycopeptides by Low-Temperature Assisted Nanopore Technique[J]. RESEARCH,2025,8:10. |
| APA | Wang, Jia-Hong.,Ma, Wenjing.,Hu, Zheng-Li.,Gao, Zhaobing.,Long, Yi-Tao.,...&Ying, Yi-Lun.(2025).Direct Identification of O-Glycopeptides by Low-Temperature Assisted Nanopore Technique.RESEARCH,8,10. |
| MLA | Wang, Jia-Hong,et al."Direct Identification of O-Glycopeptides by Low-Temperature Assisted Nanopore Technique".RESEARCH 8(2025):10. |
入库方式： OAI收割
来源：上海药物研究所
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