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Triple-Band NIR-II Fluorescent Nanoprobe-Based Lateral Flow Immunoassay Biosensor for Point-of-Care Testing of Acute Kidney Injury

文献类型：期刊论文

作者Liu, Sha3,4; Wang, Shangyun1; Suo, Yongkuan4,5; Zhang, Shanshan4; Tian, Nannan3,4; Zhao, Shuxian3,4; Wei, Tao2; Song, Zhaorui4; Cheng, Zhen3,4,5
刊名ACS APPLIED NANO MATERIALS
出版日期2025-09-26
卷号8期号:38页码:18500-18509
关键词acute kidney injury (AKI) NIR-II fluorescence rare-earth nanoparticles (RENPs) lateral flow immunoassay(LFA) cystatin C (CysC)
DOI10.1021/acsanm.5c03287
英文摘要Rapid and accurate diagnosis of early-stage acute kidney injury (AKI) is critically important for improving patients' short-term survival rates and reducing the risk of long-term kidney disease. As a prominent, fast, quantitative diagnostic tool, the fluorescent lateral flow immunoassay (LFA) has been widely applied for precise clinical diagnostics. Nevertheless, the conventional fluorescent (FL) probes are hampered by the autofluorescence interference, which constrains the sensitivity of LFA. Herein, we first designed and synthesized efficient FL nanoprobes (RENPs) with three distinct FL emission peaks across the NIR-II subregions: NIR-II (900-1100 nm), NIR-IIa (1300-1400 nm), and NIR-IIb (1500-1700 nm), and the related design strategy and the luminous mechanism were also fully discussed. Benefiting from the synergistically enhanced FL signals, low autofluorescence, and excellent photostability of the triple-band RENP probes, an advanced NIR-II LFA biosensor has been developed for the detection of Cystatin C (CysC), an AKI-sensitive biomarker. The proposed NIR-II LFA biosensor showed good linearity in the range of 0.42-7.70 mg L-1, and the limit of detection (LOD) was as low as 0.34 mg L-1, which is 3.2 times lower than the clinical cutoff value. Furthermore, it also exhibited excellent correlation (R 2 = 0.9959, n = 60) with commercial diagnostic kits (LETIA method) in clinical practicality. Overall, the developed NIR-II LFA biosensor employing the triple-band RENP probes demonstrates enhanced detection performance, encompassing high sensitivity, accuracy, and specificity, while simultaneously having characteristics of rapidity, simplicity, and low cost, which holds high potential for the early diagnosis of AKI in POCT.
资助项目Key Technology Research and Development Program of Shandong Province[2024CXPT028] ; Key R&D Program of Shandong Province, China[XDB0830000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[ZR2022QH212] ; Shandong Provincial Natural Science Foundation[SYS202205] ; Shandong Laboratory Program
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:001569346300001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/321468]  
专题国家级研究中心_原创新药研究全国重点实验室
通讯作者Wei, Tao; Song, Zhaorui; Cheng, Zhen
作者单位1.Qingdao Eighth Peoples Hosp, Clin Lab, Qingdao 266034, Peoples R China
2.Jiaozhou Cent Hosp Qingdao, Qingdao 266300, Peoples R China
3.Anhui Univ Chinese Med, Sch Pharm, Hefei 230012, Peoples R China
4.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Med, Mol Imaging Ctr, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Sha,Wang, Shangyun,Suo, Yongkuan,et al. Triple-Band NIR-II Fluorescent Nanoprobe-Based Lateral Flow Immunoassay Biosensor for Point-of-Care Testing of Acute Kidney Injury[J]. ACS APPLIED NANO MATERIALS,2025,8(38):18500-18509.
APA Liu, Sha.,Wang, Shangyun.,Suo, Yongkuan.,Zhang, Shanshan.,Tian, Nannan.,...&Cheng, Zhen.(2025).Triple-Band NIR-II Fluorescent Nanoprobe-Based Lateral Flow Immunoassay Biosensor for Point-of-Care Testing of Acute Kidney Injury.ACS APPLIED NANO MATERIALS,8(38),18500-18509.
MLA Liu, Sha,et al."Triple-Band NIR-II Fluorescent Nanoprobe-Based Lateral Flow Immunoassay Biosensor for Point-of-Care Testing of Acute Kidney Injury".ACS APPLIED NANO MATERIALS 8.38(2025):18500-18509.

入库方式： OAI收割

来源：上海药物研究所

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