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  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 国家级研究中心
  5. 原创新药研究全国重点实验室
A robust platform streamlining aromatic noncanonical amino acid biosynthesis and genetic code expansion in Escherichia coli

文献类型：期刊论文

作者Zhang, Jingxuan1,7; Yu, Keying6,7; Xu, Yali5; Zhao, Wushuang4,7; Li, Yulian4,7; Wang, Ying1; Seebeck, Florian P.3; Chen, Xiao-Hua2,5; Liao, Cangsong4,6,7
刊名NATURE COMMUNICATIONS
出版日期2025-09-29
卷号16期号:1页码:13
DOI10.1038/s41467-025-63679-6
英文摘要Genetic code expansion (GCE) has significantly enhanced the diversity of proteins in the biological world, leading to a wide range of applications. Despite the advances in GCE, the cost of noncanonical amino acids (ncAAs) remains one of the major obstacles for large-scale production. In situ biosynthesis of ncAAs from commercial precursors offers a promising solution to this challenge, yet only a few biosynthetic pathways have been reported. Here, we present a platform that couples the biosynthesis of aromatic ncAAs with genetic code expansion in E. coli, enabling the production of proteins and peptides containing ncAAs. Forty ncAAs are synthesized from aryl aldehydes by the biosynthetic pathway, while nineteen ncAAs are incorporated into superfolder GFP using three orthogonal translation systems. The platform's versatility is demonstrated by the production of macrocyclic peptides and antibody fragments. We envision that the platform will facilitate the production of peptides, enzymes, and antibody fragments containing ncAAs.
WOS关键词SUBSTRATE-SPECIFICITY ; ASPARTATE ; AMINOTRANSFERASE ; MUTANT ; CELLS
资助项目National Natural Science Foundation of China (National Science Foundation of China)[22071259] ; National Natural Science Foundation of China (National Science Foundation of China)[22307126] ; National Natural Science Foundation of China (NSFC)[206039] ; Swiss National Science Foundation[XDB1060000] ; Strategic Priority Research Program of the Chinese Academy of Sciences[SIMM0220233001] ; Shanghai Institute of Materia Medica ; Chinese Academy of Sciences
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:001586153800031
出版者NATURE PORTFOLIO
源URL[http://119.78.100.183/handle/2S10ELR8/321609]  
专题国家级研究中心_原创新药研究全国重点实验室
通讯作者Chen, Xiao-Hua; Liao, Cangsong
作者单位1.Tianjin Univ, State Key Lab Synthet Biol, Tianjin, Peoples R China
2.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou, Peoples R China
3.Univ Basel, Dept Chem, Basel, Switzerland
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China
6.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing, Peoples R China
7.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Jingxuan,Yu, Keying,Xu, Yali,et al. A robust platform streamlining aromatic noncanonical amino acid biosynthesis and genetic code expansion in Escherichia coli[J]. NATURE COMMUNICATIONS,2025,16(1):13.
APA Zhang, Jingxuan.,Yu, Keying.,Xu, Yali.,Zhao, Wushuang.,Li, Yulian.,...&Liao, Cangsong.(2025).A robust platform streamlining aromatic noncanonical amino acid biosynthesis and genetic code expansion in Escherichia coli.NATURE COMMUNICATIONS,16(1),13.
MLA Zhang, Jingxuan,et al."A robust platform streamlining aromatic noncanonical amino acid biosynthesis and genetic code expansion in Escherichia coli".NATURE COMMUNICATIONS 16.1(2025):13.

入库方式： OAI收割

来源：上海药物研究所

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