The B169L protein of African swine fever virus functions as a viroporin that activates the calcium-mediated inflammasome
文献类型：期刊论文
| 作者 | Li, Jiaqi4; Li, Qiguang3; Wang, Yanjin4; Guo, Zhanhao4; Qu, Yuxin4; Wang, Xiao2; Deng, Hao4; Dai, Jingwen4; Li, Lian-Feng4; He, Wen-Rui1 |
| 刊名 | PLOS PATHOGENS
 |
| 出版日期 | 2025-11-01 |
| 卷号 | 21期号:11页码:26 |
| ISSN号 | 1553-7366 |
| DOI | 10.1371/journal.ppat.1013686 |
| 英文摘要 | African swine fever (ASF) is a highly contagious and often fatal viral disease caused by African swine fever virus (ASFV), which poses a significant economic burden on the global pig industry. ASFV infection triggers a robust production of proinflammatory cytokines, leading to severe inflammation that contributes significantly to the high mortality rate associated with ASF. However, the underlying mechanisms remain incompletely understood. Here, we identified the ASFV B169L protein (pB169L) as a viroporin that exerts dual functions in viral replication and proinflammatory responses. We demonstrated that pB169L formed oligomeric calcium (Ca2+)-permeable channels in vitro by bilayer lipid membrane assay. The ectopically expressed pB169L significantly altered Ca2+ homeostasis in cells and induced robust proinflammatory responses. Mutagenesis revealed critical residues-including P29, K55, and K57-that are indispensable for channel function and proinflammatory signaling. Importantly, the B169L gene knockdown during ASFV infection reduced inflammasome activation and viral replication, highlighting its dual role as both a structural component of virus and an inflammatory mediator. These findings provide the first direct evidence that ASFV encodes a functional viroporin and uncover a novel mechanism by which ASFV manipulates Ca2+ homeostasis to drive inflammasome activation, offering new insights into ASFV pathogenesis and potential antiviral targets. |
| WOS关键词 | INHIBITOR ; REPLICATION ; TRIGGERS ; RELEASE ; NLRP3 |
| 资助项目 | National Key R&D Program of China[2021YFD1800104] ; Natural Science Foundation of China[32473002] ; Heilongjiang Provincial Natural Science Foundation of China[TD2023C007] |
| WOS研究方向 | Microbiology ; Parasitology ; Virology |
| 语种 | 英语 |
| WOS记录号 | WOS:001616906800001 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321738]  |
| 专题 | 国家级研究中心_原创新药研究全国重点实验室 |
| 通讯作者 | Gao, Zhaobing; Xia, Bingqing; Li, Su; Qiu, Hua-Ji |
| 作者单位 | 1.Henan Agr Univ, Coll Vet Med, Int Joint Res Ctr Natl Anim Immunol, Zhengzhou, Henan, Peoples R China 2.Binzhou Med Univ, Sch Basic Med Sci, Yantai, Shandong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, State Key Lab Drug Res, Shanghai, Peoples R China 4.Chinese Acad Agr Sci, State Key Lab Anim Dis Prevent & Control, Natl African Swine Fever Para Reference Lab, Harbin Vet Res Inst,Natl High Containment Facil An, Harbin, Heilongjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jiaqi,Li, Qiguang,Wang, Yanjin,et al. The B169L protein of African swine fever virus functions as a viroporin that activates the calcium-mediated inflammasome[J]. PLOS PATHOGENS,2025,21(11):26. |
| APA | Li, Jiaqi.,Li, Qiguang.,Wang, Yanjin.,Guo, Zhanhao.,Qu, Yuxin.,...&Qiu, Hua-Ji.(2025).The B169L protein of African swine fever virus functions as a viroporin that activates the calcium-mediated inflammasome.PLOS PATHOGENS,21(11),26. |
| MLA | Li, Jiaqi,et al."The B169L protein of African swine fever virus functions as a viroporin that activates the calcium-mediated inflammasome".PLOS PATHOGENS 21.11(2025):26. |
入库方式： OAI收割
来源：上海药物研究所
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