Integrative Dynamic Proteomics and Acyl-Proteomics Analyses in Clostridium butyricum
文献类型：期刊论文
| 作者 | Li, Guorong4,5; Yang, Qiong4,5; Liu, Xia5; Tang, Yanqing5; Tian, Nana1,5; Wang, Danfeng1,5; Zhu, Xinping2,5; Ren, Bingjie4,5; Tu, Zhijie2,5; Chen, Yan4 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2025-11-12 |
| 页码 | 17 |
| 关键词 | |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.5c00492 |
| 英文摘要 | Clostridium butyricum (C. butyricum) is a notable butyrate-producing intestinal probiotic that has been utilized for the enhancement and treatment of various intestinal and extraintestinal diseases. Butyrate and acetate, as significant metabolites of this bacterium, are short-chain fatty acids (SCFAs) that play crucial roles in maintaining intestinal stability and safeguarding host health. However, the regulatory functions of lysine butyrylation (Kbu) and acetylation (Kac), mediated by these metabolites, remain inadequately understood. In this study, we performed a comprehensive dynamic multiomic analysis encompassing protein expression, Kbu, and Kac in C. butyricum. Our multiomic analysis identified a total of 2622 proteins, 1887 Kbu sites, and 2188 Kac sites. Subsequent bioinformatic analyses revealed that biological functions such as gene expression and energy metabolism exhibited dynamic changes throughout the growth cycle at both the proteome and post-translational modification levels. Enzymatic experiments demonstrated that Kbu modified key active sites of important substrates, including alanine-tRNA ligase at K794, GMP synthase at K384, and probable butyrate kinase at K269. This study enhances the current understanding of lysine acylations in bacteria and focuses on elucidating the finely tuned regulatory mechanisms of Kbu during the growth and development of C. butyricum. |
| WOS关键词 | ACETYLATION ; METABOLISM ; LANDSCAPE ; DIVERSITY |
| 资助项目 | Guangdong Provincial Introduction of Innovative Research and Development Team[2019B090904008] ; Innovative Research Team of High-level Local University in Shanghai[NA] ; Program of Shanghai Academic Research Leader[22XD1420900] ; National Natural Science Foundation of China[32322048] ; Youth Innovation Promotion Association[CAS2021276] ; Shanghai Rising-Star Program[22QA1411100] ; National Key Research and Development Program of China[2023YFA1800400] ; Zhongshan Science and Technology Bureau[CXTD2024006] ; China Association for Science and Technology[2022QNRC001] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001614168200001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/321753]  |
| 专题 | 国家级研究中心_原创新药研究全国重点实验室 |
| 通讯作者 | Tan, Minjia; Xu, Jun-Yu |
| 作者单位 | 1.Zunyi Med Univ, Sch Pharm, Zhuhai 519041, Peoples R China 2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 4.Guizhou Med Univ, Sch Pharmaceut Sci, State Key Lab Discovery & Utilizat Funct Component, Guiyang 561113, Guizhou, Peoples R China 5.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528454, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guorong,Yang, Qiong,Liu, Xia,et al. Integrative Dynamic Proteomics and Acyl-Proteomics Analyses in Clostridium butyricum[J]. JOURNAL OF PROTEOME RESEARCH,2025:17. |
| APA | Li, Guorong.,Yang, Qiong.,Liu, Xia.,Tang, Yanqing.,Tian, Nana.,...&Xu, Jun-Yu.(2025).Integrative Dynamic Proteomics and Acyl-Proteomics Analyses in Clostridium butyricum.JOURNAL OF PROTEOME RESEARCH,17. |
| MLA | Li, Guorong,et al."Integrative Dynamic Proteomics and Acyl-Proteomics Analyses in Clostridium butyricum".JOURNAL OF PROTEOME RESEARCH (2025):17. |
入库方式： OAI收割
来源：上海药物研究所
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