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BfrR-mediated biofilm regulation in the seafood-borne pathogen Vibrio parahaemolyticus

文献类型：期刊论文

作者Sun, Hui2,3; Zhang, Miaomiao3; Wang, Jiancheng1; Xiong, Shuhui2,3; Li, Xue3; Luo, Xi3; Yang, Chao1; Lu, Renfei2,3; Zhang, Yiquan2,3
刊名FOOD RESEARCH INTERNATIONAL
出版日期2025-12-01
卷号222页码:11
关键词Vibrio parahaemolyticus BfrR Biofilm Regulation Transcriptome Vibrio parahaemolyticus BfrR Biofilm Regulation C-di-GMP Transcriptome
ISSN号0963-9969
DOI10.1016/j.foodres.2025.117792
通讯作者Lu, Renfei(rainman78@163.com) ; Zhang, Yiquan(zhangyiquanq@163.com)
英文摘要Vibrio parahaemolyticus is a major seafood-associated pathogen whose biofilm formation enhances environmental persistence, antibiotic resistance, and food safety risks. In this study, we identified a LysR-type regulator, BfrR (VPA1687), as a critical mediator of biofilm development in V. parahaemolyticus. Deletion of bfrR severely impaired biofilm formation, switching colony morphology from wrinkled to smooth and reducing extracellular matrix components (exopolysaccharides (EPS), proteins, and extracellular DNA). BfrR also modulated metabolic activity in biofilm-embedded cells and exhibited dual control over motility: it inhibited swimming while promoting swarming. Mechanistically, BfrR elevated intracellular c-di-GMP levels by regulating genes encoding GGDEF/EAL domain proteins. RNA-seq revealed BfrR influences 500 genes, including biofilm-related pathways (e.g., cps operon for EPS), virulence factors, and flagellar genes. Critically, BfrR was essential for biofilm formation on diverse food-contact surfaces (shrimp, crab, stainless steel, plastic, and glass). These findings establish BfrR as a global regulator that coordinates biofilm formation, motility, and c-di-GMP signaling, highlighting its potential as a target for mitigating V. parahaemolyticus biofilm-associated risks in the food industry.
WOS关键词VIRULENCE ; MOTILITY ; SEQUENCE ; DATABASE ; PROTEIN ; GENES ; APHA
资助项目Natural Science Foundation of Nantong[JC2023045]
WOS研究方向Food Science & Technology
语种英语
WOS记录号WOS:001616145600002
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/321974]  
专题中国科学院上海药物研究所
通讯作者Lu, Renfei; Zhang, Yiquan
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 200031, Peoples R China
2.Nantong Univ, Sch Med, Nantong 226001, Jiangsu, Peoples R China
3.Nantong Univ, Nantong Peoples Hosp 3, Affiliated Nantong Hosp 3, Dept Clin Lab, Nantong 226006, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Hui,Zhang, Miaomiao,Wang, Jiancheng,et al. BfrR-mediated biofilm regulation in the seafood-borne pathogen Vibrio parahaemolyticus[J]. FOOD RESEARCH INTERNATIONAL,2025,222:11.
APA Sun, Hui.,Zhang, Miaomiao.,Wang, Jiancheng.,Xiong, Shuhui.,Li, Xue.,...&Zhang, Yiquan.(2025).BfrR-mediated biofilm regulation in the seafood-borne pathogen Vibrio parahaemolyticus.FOOD RESEARCH INTERNATIONAL,222,11.
MLA Sun, Hui,et al."BfrR-mediated biofilm regulation in the seafood-borne pathogen Vibrio parahaemolyticus".FOOD RESEARCH INTERNATIONAL 222(2025):11.

入库方式： OAI收割

来源：上海药物研究所

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