Systematic comparison of the effects of various fixatives on the stability of the cellular proteome
文献类型：期刊论文
| 作者 | Chen, Yulu3; Zou, Xun3; Wang, Lulu1,2; Gao, Yuan1,2; Fu, Hang1; Liu, Bin3; Jiang, Haowen5; Tan, Minjia2,3,4,5; Zhai, Linhui4 |
| 刊名 | JOURNAL OF PROTEOMICS
 |
| 出版日期 | 2026-01-20 |
| 卷号 | 323页码:13 |
| 关键词 | Proteomics Fixatives Proteome stability Proteome identification Mass spectrometry analysis |
| ISSN号 | 1874-3919 |
| DOI | 10.1016/j.jprot.2025.105567 |
| 英文摘要 | Proteomics research represents a critical area within modern biomedical science. Fixatives, as essential components in proteomic analysis, play a pivotal role in maintaining proteome stability and analytical accuracy, while also exerting a substantial influence on experimental outcomes. However, there remains a limited understanding of how various fixatives affect proteome stability. In this study, we conducted a systematic comparison of commonly used fixation agents-specifically formaldehyde, paraformaldehyde, methanol, and ethanol-to evaluate how they affect the stability of the cellular proteome. Our results show that different fixatives lead to notably different levels of proteome stability. Importantly, formaldehyde solution and paraformaldehyde produce cross-linking effects that can significantly reduce protein solubility and hinder the efficiency of enzymatic digestion. Additionally, we analyzed the proteomic profiles of both drug-treated and untreated samples under different fixation conditions to better understand how these conditions may affect the quality and interpretation of mass spectrometry data. These results provide a scientific basis for selecting the most appropriate fixative in proteomics research. Our study also contributes to improving the compatibility between fixatives and mass spectrometry technologies, offering dependable technical support for biological and biomedical research. Significance: We conducted a systematic comparison of commonly used fixation agents-specifically formaldehyde, paraformaldehyde, methanol, and ethanol-to evaluate how they affect the stability of the cellular proteome. These results provide a scientific basis for selecting the most appropriate fixative in proteomics research. Our study also contributes to improving the compatibility between fixatives and mass spectrometry technologies, offering dependable technical support for biological and biomedical research. |
| WOS关键词 | MASS-SPECTROMETRY |
| 资助项目 | National Natural Science Foundation of China[32171434] ; National Natural Science Foundation of China[22577093] ; National Natural Science Foundation of China[22225702] ; Program of Shanghai Academic Research Leader[22XD1420900] ; State Key Laboratory of Drug Research[SIMM2105KF-13] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001622595000001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322022]  |
| 专题 | 国家级研究中心_原创新药研究全国重点实验室 |
| 通讯作者 | Zhai, Linhui |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Sch Pharm, Nanjing 210023, Jiangsu, Peoples R China 3.Jiangsu Ocean Univ, Coll Pharm, Jiangsu Key Lab Marine Pharmaceut Cpd Screening, Lianyungang 222005, Peoples R China 4.Tongji Univ, Shanghai Peoples Hosp 4, Translat Res Inst Brain & Brain Like Intelligence, Sch Med, Shanghai 200434, Peoples R China 5.Fudan Univ, Huashan Hosp, Dept Urol, Shanghai 200040, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Yulu,Zou, Xun,Wang, Lulu,et al. Systematic comparison of the effects of various fixatives on the stability of the cellular proteome[J]. JOURNAL OF PROTEOMICS,2026,323:13. |
| APA | Chen, Yulu.,Zou, Xun.,Wang, Lulu.,Gao, Yuan.,Fu, Hang.,...&Zhai, Linhui.(2026).Systematic comparison of the effects of various fixatives on the stability of the cellular proteome.JOURNAL OF PROTEOMICS,323,13. |
| MLA | Chen, Yulu,et al."Systematic comparison of the effects of various fixatives on the stability of the cellular proteome".JOURNAL OF PROTEOMICS 323(2026):13. |
入库方式： OAI收割
来源：上海药物研究所
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