Spatiotemporal oxygen modulation reprograms hypoxia-induced antibiotic resistance
文献类型：期刊论文
| 作者 | Shi, Shuo3; Bai, Ma3; Kong, Lili3,4; Li, Jinjie3; Ding, Xiyan3; Yang, Jialun3; Wang, Jianlong2; Guo, Chao1; Duan, Jinyou3 |
| 刊名 | CHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2025-12-01 |
| 卷号 | 525页码:14 |
| 关键词 | Hypoxia-induced antibiotic resistance Bilayered microneedle Chlorella Biofilm Spatiotemporal oxygen modulation |
| ISSN号 | 1385-8947 |
| DOI | 10.1016/j.cej.2025.170595 |
| 通讯作者 | Wang, Jianlong(wanglong79@nwsuaf.edu.cn) ; Guo, Chao(guochao@xzhmu.edu.cn) ; Duan, Jinyou(jduan@nwsuaf.edu.cn) |
| 英文摘要 | Hypoxia-driven antibiotic resistance and persistent biofilm infections are critical barriers to the healing of chronic wounds, creating an urgent need for microenvironment-modulating therapeutic strategies. Here, we develop a spatiotemporally controlled oxygen modulation strategy using a bilayered microneedle system to reprogram hypoxia-induced antibiotic resistance. The system features differential release kinetics, with an inner hyaluronic acid (HA) layer enabling rapid antibiotic release (98.1 % within 30 min) and an outer methacrylated hyaluronic acid (HAMA) layer incorporating Chlorella for sustained photosynthetic oxygen generation (7.46 mg L- 1 dissolved oxygen). This dual-modality approach significantly recovers antibiotic sensitivity, reducing the minimum biofilm eradication concentration (MBEC) against P. aeruginosa by 32-fold for commercial ceftazidime (from 2048 mu g/mL to 64 mu g/mL) through concurrent suppression of quorum sensing and drug efflux pump expression. In a biofilm-infected subcutaneous abscess model which recapitulated human chronic infection features with irregular ulcer border, TChl@MN + light achieved effective bacterial clearance at 7 days, suppressed inflammation (lower IL-6, higher IL-10), promoted M2 polarization (elevated CD163 cells), and boosted collagen deposition with negligible toxicity. Thus, this spatiotemporally controlled oxygen modulation strategy restores antibiotic susceptibility by targeting the hypoxic biofilm microenvironment, offering a sustainable alternative to traditional antibiotic therapies for chronic infected wound treatment. |
| WOS关键词 | STAPHYLOCOCCUS-AUREUS ; BIOFILM |
| 资助项目 | National Science Foundation of China[82304735] ; Shaanxi Province postdoctoral research project[2023BSHYDZZ67] ; Macau Young Scholars Program[AM2022021] ; Open Project of Qinghai Key Laboratory of Qinghai-Tibet Plateau Biological Resources |
| WOS研究方向 | Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:001619485000034 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322088]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Wang, Jianlong; Guo, Chao; Duan, Jinyou |
| 作者单位 | 1.Xuzhou Med Univ, Sch Pharm, Xuzhou 221004, Jiangsu, Peoples R China 2.Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Shaanxi, Peoples R China 3.Northwest A&F Univ, Coll Chem & Pharm, Yangling 712100, Shaanxi, Peoples R China 4.Chinese Acad Sci, Zhongshan Inst Drug Discovery ZIDD, Shanghai Inst Mat Med, Zhongshan 528400, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Shuo,Bai, Ma,Kong, Lili,et al. Spatiotemporal oxygen modulation reprograms hypoxia-induced antibiotic resistance[J]. CHEMICAL ENGINEERING JOURNAL,2025,525:14. |
| APA | Shi, Shuo.,Bai, Ma.,Kong, Lili.,Li, Jinjie.,Ding, Xiyan.,...&Duan, Jinyou.(2025).Spatiotemporal oxygen modulation reprograms hypoxia-induced antibiotic resistance.CHEMICAL ENGINEERING JOURNAL,525,14. |
| MLA | Shi, Shuo,et al."Spatiotemporal oxygen modulation reprograms hypoxia-induced antibiotic resistance".CHEMICAL ENGINEERING JOURNAL 525(2025):14. |
入库方式： OAI收割
来源：上海药物研究所
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