Recent Advances in On-Tissue Chemical Derivatization Strategies for Enhancing MALDI-MSI
文献类型：期刊论文
| 作者 | Huang, Meng1,6; Qi, Xiaoyu1,5; Zhu, Dafu1; Zhou, Hao1; Yuan, Jie1; Misic, Danijela4; Sokovic, Marina4; Xu, Hongxi6; Sun, Lu5; Ye, Yang1,3,6 |
| 刊名 | MASS SPECTROMETRY REVIEWS
 |
| 出版日期 | 2025-12-03 |
| 页码 | 24 |
| 关键词 | functional group targeting ionization enhancement MALDI mass spectrometry imaging on-tissue chemical derivatization spatial metabolomics |
| ISSN号 | 0277-7037 |
| DOI | 10.1002/mas.70016 |
| 通讯作者 | Xu, Hongxi(xuhongxi88@gmail.com) ; Sun, Lu(sunlu@vip.126.com) ; Ye, Yang(yye@simm.ac.cn) ; Liu, Jia(jia.liu@simm.ac.cn) |
| 英文摘要 | Matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has rapidly advanced in biomedical research, enabling label-free, untargeted spatial detection of metabolites, lipids, proteins, and glycans in tissue sections. However, challenges such as low ionization efficiency and chemical instability limit the detection of certain molecules. To address these issues, on-tissue chemical derivatization (OTCD) has been widely applied as an effective strategy to enhance imaging capabilities. This review systematically summarizes the development of derivatization reagents targeting different reactive functional groups and their applications in MALDI-MSI, including strategies for the derivatization of amines, carbonyls, carboxyls, double bonds, hydroxyls, thiols, and platinum-based drugs. Particular attention is given to how these derivatization reagents enhance the detection range and biological relevance by increasing molecular weight, improving ionization efficiency, and reducing background noise interference. Additionally, we explore the application of OTCD in various biological samples and discuss challenges related to experimental workflows, derivatization efficiency, and tissue integrity. This review provides important theoretical support for the advancement of MSI technology and highlights its broad potential applications in biomedical research. |
| WOS关键词 | IMAGING MASS-SPECTROMETRY ; PROTEIN IDENTIFICATION ; CARBONYL-COMPOUNDS ; MATRIX ; CANCER ; METABOLITES ; NEUROTRANSMITTERS ; REAGENTS ; ACIDS |
| 资助项目 | National Natural Science Foundation of China[82373827] ; National Key R&D Program Strategic Scientific and Technological Innovation Cooperation Key Project[2022YFE0203600] ; National Key R&D Program Strategic Scientific and Technological Innovation Cooperation Key Project[2024YFE0117200] ; Ministry of Science and Technology of China |
| WOS研究方向 | Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:001630055200001 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322175]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Xu, Hongxi; Sun, Lu; Ye, Yang; Liu, Jia |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 2.Univ Chinese Acad Sci, Sch Pharmaceut Sci & Technol, Hangzhou Inst Adv Study, Hangzhou, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, China Serbia Belt & Rd Joint Lab Nat Prod & Drug D, Shanghai, Peoples R China 4.Inst Biol Res Sinisa Stankov, China Serbia Belt & Rd Joint Lab Nat Prod & Drug D, Belgrade, Serbia 5.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang, Peoples R China 6.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Meng,Qi, Xiaoyu,Zhu, Dafu,et al. Recent Advances in On-Tissue Chemical Derivatization Strategies for Enhancing MALDI-MSI[J]. MASS SPECTROMETRY REVIEWS,2025:24. |
| APA | Huang, Meng.,Qi, Xiaoyu.,Zhu, Dafu.,Zhou, Hao.,Yuan, Jie.,...&Liu, Jia.(2025).Recent Advances in On-Tissue Chemical Derivatization Strategies for Enhancing MALDI-MSI.MASS SPECTROMETRY REVIEWS,24. |
| MLA | Huang, Meng,et al."Recent Advances in On-Tissue Chemical Derivatization Strategies for Enhancing MALDI-MSI".MASS SPECTROMETRY REVIEWS (2025):24. |
入库方式： OAI收割
来源：上海药物研究所
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