GH28 polygalacturonase of human gut Bacteroides spp. confers the degradation of a novel branched galacturonan isolated from Jasminum nudiflorum
文献类型：期刊论文
| 作者 | Li, Yun2,3; Hu, Tingmei1,4; Li, Meixia2,3; Chen, Huixian1,5; Duo, Lana3; Jin, Can1,3; Ding, Kan1,2,3,4,5 |
| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2026-03-01 |
| 卷号 | 375页码:15 |
| 关键词 | Jasminum nudiflorum Human gut microbiota Bacteroides spp. Degradation Polygalacturonase |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2025.124745 |
| 通讯作者 | Li, Meixia(limei@simm.ac.cn) ; Jin, Can(jincan@zidd.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | The specific enzymes responsible for metabolizing distinct pectin structures have not been fully elucidated due to the structural diversity of pectin. Thus, we hypothesize that there may be other enzymes to target pectin. Here, a novel pectic polysaccharide CFM224 (18.2 kDa) was isolated from Jasminum nudiflorum for the first time. Structural analysis revealed a unique structure comprising branched galacturonan backbone interspersed with 2-alpha-Rhap, while 2, 4-alpha-Rhap existed on the branch. Complex branches including arabinogalactan, glucan polymers and t-GalpA, Delta HexpA, the other was composed of 4-beta-Glcp, 2, 4-alpha-Rhap, t-beta-Rhap and t-GalpA. CFM224 promoted the growth of Bacteroides spp. and underwent degradation. Furthermore, two polygalacturonases belonging to the glycoside hydrolase 28 (GH28) family, which degraded CFM224 into GalA, encoded by BXY_32940 and BT_4123 genes of Bacteroides spp., respectively, were identified. Notably, the predicted structures of BXY_32940GH28 and BT_4123GH28 exhibit significant homology to rhamnogalacturonase. Site mutation and enzymatic activity analysis verified that D312 might be the catalytic residue of GH28. Genetic analysis demonstrated that polygalacturonase was predominantly encoded by Bacteroides spp. Overall, these findings further expand the metabolic network of pectin and may provide novel candidates for prebiotic screening. |
| WOS关键词 | POLYSACCHARIDE UTILIZATION ; PECTIN ; METHYLATION ; L. ; AGGREGATION ; ENZYME ; FRUITS ; ACID |
| 资助项目 | National Key Research and Development Program of China[2022YFA1303802] ; Shanghai Municipal Science and Technology Major Project, National Natural Science Foundation of China[32271332] ; Shanghai Municipal Science and Technology Major Project, National Natural Science Foundation of China[31870801] ; Shanghai Municipal Science and Technology Major Project, National Natural Science Foundation of China[82341097] ; Shanghai Municipal Science and Technology Major Project, National Natural Science Foundation of China[32471328] ; Shanghai Public health special Project[2024GKM19] ; Shanghai Post-doctoral Excellence Program[20226860] ; High-level New R D Institute[2019B090904008] ; Department of Science and Technology of Guangdong Province[2021B0909050003] ; Ninth Batch of Innovative Scientific Research Team Projects from Zhongshan Science and Technology Bureau |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001632125600001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322339]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Meixia; Jin, Can; Ding, Kan |
| 作者单位 | 1.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, SSIP Healthcare & Med Demonstrat Zone, Zhongshan 528400, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Based Drug Res Ctr, CAS Key Lab Receptor Res, Shanghai 201203, Peoples R China 4.Guizhou Med Univ, Sch Pharmaceut Sci, State Key Lab Discovery & Utilizat Funct Component, 6 Ankang Ave, Anshun 561113, Guizhou, Peoples R China 5.Southern Med Univ, Sch Pharmaceut Sci, 1023 South Shatai Rd, Guangzhou 510515, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yun,Hu, Tingmei,Li, Meixia,et al. GH28 polygalacturonase of human gut Bacteroides spp. confers the degradation of a novel branched galacturonan isolated from Jasminum nudiflorum[J]. CARBOHYDRATE POLYMERS,2026,375:15. |
| APA | Li, Yun.,Hu, Tingmei.,Li, Meixia.,Chen, Huixian.,Duo, Lana.,...&Ding, Kan.(2026).GH28 polygalacturonase of human gut Bacteroides spp. confers the degradation of a novel branched galacturonan isolated from Jasminum nudiflorum.CARBOHYDRATE POLYMERS,375,15. |
| MLA | Li, Yun,et al."GH28 polygalacturonase of human gut Bacteroides spp. confers the degradation of a novel branched galacturonan isolated from Jasminum nudiflorum".CARBOHYDRATE POLYMERS 375(2026):15. |
入库方式： OAI收割
来源：上海药物研究所
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