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Modulation of Core-Fucosylation Oxidation for Selective Enrichment and Characterization of Cell Surface Glycoprotein

文献类型：期刊论文

作者Guo, Xin1,2; Liu, Xiaoyan2,3; Fang, Zheng2,3; Wang, Yan2; Liu, Lei2,3; Wang, Zhongyu2,3; Tang, Feng4; Huang, Wei4; Deng, Zhenpeng2; Wang, Qi2
刊名ACS CHEMICAL BIOLOGY
出版日期2025-12-19
卷号20期号:12页码:2979-2990
ISSN号1554-8929
DOI10.1021/acschembio.5c00762
通讯作者Dong, Mingming(dongmm@dlut.edu.cn)
英文摘要Protein core-fucosylation plays a crucial role in regulating cell surface protein functions and is involved in various biological processes, including cell signaling, immune response, and cancer progression. However, core-fucosylation (CF) poses particular challenges in identification and characterization due to its low abundance and the high heterogeneity of N-glycosylation. To overcome these challenges, this study systematically investigated the oxidation mechanisms of core-fucose and developed a selective enrichment strategy that combines chemical oxidation with glycan truncation for cell surface core-fucosylation characterization. Specifically, sodium periodate was employed to selectively oxidize cell surface glycans. In combination with the endoglycosidases Endo M and Endo F3, which possessed complementary substrate specificity and broad tolerance, this approach efficiently truncated N-glycans, leaving core-fucosylated glycopeptides bearing aldehyde tags. Utilizing reversible hydrazide chemistry, core-fucosylated glycopeptides were selectively enriched. The developed strategy was applied to profile cell surface core-fucosylated protein in HeLa cells, which yielded 74 core-fucosylated glycopeptides corresponding to 21 key cell surface drug targets, thereby validating the efficacy of this approach. Collectively, this study systematically investigated the mechanism of core-fucosylation oxidation and developed a new technical tool for studying cell surface protein core-fucosylation.
WOS关键词HIGH-MANNOSE ; CANCER ; FUCOSE ; GLYCOSYLATION ; EXPRESSION ; AFFINITY ; IDENTIFICATION ; ANTIBODY ; MARKER ; LECTIN
资助项目Fundamental Research Funds for the Central Universities[DUT25LAB106] ; National Natural Science Foundation of China[22034007] ; National Key Research and Development Program of China[2024YFA1306300]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:001634108800001
出版者AMER CHEMICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/322342]  
专题中国科学院上海药物研究所
通讯作者Dong, Mingming
作者单位1.Dalian Univ Technol, Sch Bioengn, MOE Key Lab Biointelligent Mfg, Dalian 116000, Liaoning, Peoples R China
2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, State Key Lab Med Prote,CAS Key Lab Separat Sci An, Dalian 116023, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Materia Med, CAS Ctr Excellence Mol Cell Sci, Ctr Biotherapeut Discovery Res,CAS Key Lab Recepto, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Guo, Xin,Liu, Xiaoyan,Fang, Zheng,et al. Modulation of Core-Fucosylation Oxidation for Selective Enrichment and Characterization of Cell Surface Glycoprotein[J]. ACS CHEMICAL BIOLOGY,2025,20(12):2979-2990.
APA Guo, Xin.,Liu, Xiaoyan.,Fang, Zheng.,Wang, Yan.,Liu, Lei.,...&Dong, Mingming.(2025).Modulation of Core-Fucosylation Oxidation for Selective Enrichment and Characterization of Cell Surface Glycoprotein.ACS CHEMICAL BIOLOGY,20(12),2979-2990.
MLA Guo, Xin,et al."Modulation of Core-Fucosylation Oxidation for Selective Enrichment and Characterization of Cell Surface Glycoprotein".ACS CHEMICAL BIOLOGY 20.12(2025):2979-2990.

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来源：上海药物研究所

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