A structurally defined galactoglucan from Arisaema erubescens with a multi-target tumor-associated protein binding domain
文献类型：期刊论文
| 作者 | Zhu, Yue1,2,3,4; Jin, Can2; Ding, Kan1,2,3,4 |
| 刊名 | CARBOHYDRATE RESEARCH
 |
| 出版日期 | 2026-02-01 |
| 卷号 | 560页码:12 |
| 关键词 | Polysaccharide structure Multi-target binding Antitumor potential alpha-Glucosidase resistance Arisaema erubescens (Wall.) Schott |
| ISSN号 | 0008-6215 |
| DOI | 10.1016/j.carres.2025.109781 |
| 通讯作者 | Jin, Can(jincan@zidd.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | As targeted therapy assumes an increasingly pivotal role in comprehensive cancer treatment, there is a growing imperative to develop natural drugs that exhibit low toxicity and minimal side effects. Polysaccharides derived from the traditional Chinese herbal medicine Arisaema erubescens have been reported to show antitumor potential, however the key structural features and specific molecular targets responsible for their pharmacological effects are still vague. To address this question, a homogeneous polysaccharide TNX05 (Mw approximate to 9 kDa) was obtained and characterized from Arisaema erubescens. Structural analysis suggested that TNX05 was a galactoglucan with a backbone of 1, 4-alpha-D-Glcp, 1, 4-(3-D-Glcp, and 1, 3-(3-D-Galp residues, with side chains--*1-alpha/(3-D-Glcp-(6-* 1)-alpha-D-Glcp and-*1)-alpha-D-Glcp-substituted at the C-4 and C-6 of the 1, 4-alpha-D-Glcp units, respectively. Combining enzymatic hydrolysis, molecular docking, and protein-binding assays we showed a key core domain (TNX05II), which exhibited micromolar-range binding affinity for ten tumor-associated targets: Glypican-6 (GPC-6), glucuronic acid epimerase (Glce), S100 calcium-binding protein A4 (S100A4), S100A6, nucleoside diphosphate kinase 1 (NME1), fibroblast growth factor 17 (FGF17), proto-oncogene tyrosine-protein kinase Src (SRC), kelch-like ECH-associated protein 1 (KEAP1), Galectin-3 (Gal-3), and protein phosphatase 3 catalytic subunit alpha (PPP3CA). Additionally, TNX05II was significantly resistant to alpha-glucosidase degradation. This study suggests a possible key structure-target relationship underlying TNX05's antitumor activity, providing a molecular basis for the antitumor mechanism of Arisaema polysaccharides. |
| WOS关键词 | POLYSACCHARIDES ; GLUCANS |
| 资助项目 | Shanghai Post-doctoral Excellence Program[2022686] ; Zhongshan Active structural domains, targeting, functional mechanisms of polysaccharides and their creation of new anti-tumor drugs Project[CXTD2022012] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001636463900001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322375]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Jin, Can; Ding, Kan |
| 作者单位 | 1.Henan Univ Chinese Med, Acad Chinese Med Sci, Zhengzhou 450046, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, SSIP Healthcare & Med Demonstrat Zone, Zhongshan 528400, Guangdong, Peoples R China 3.Henan Key Lab Chinese Med Polysaccharides & Drugs, Zhengzhou 450046, Peoples R China 4.Henan Polysaccharide Res Ctr, Zhengzhou 450046, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Yue,Jin, Can,Ding, Kan. A structurally defined galactoglucan from Arisaema erubescens with a multi-target tumor-associated protein binding domain[J]. CARBOHYDRATE RESEARCH,2026,560:12. |
| APA | Zhu, Yue,Jin, Can,&Ding, Kan.(2026).A structurally defined galactoglucan from Arisaema erubescens with a multi-target tumor-associated protein binding domain.CARBOHYDRATE RESEARCH,560,12. |
| MLA | Zhu, Yue,et al."A structurally defined galactoglucan from Arisaema erubescens with a multi-target tumor-associated protein binding domain".CARBOHYDRATE RESEARCH 560(2026):12. |
入库方式： OAI收割
来源：上海药物研究所
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