Notoginsenoside R1 mitigates UVB-induced skin sunburn injury through modulation of N4-acetylcytidine and autophagy
文献类型：期刊论文
| 作者 | Liang, Shuyun1,4,6; Liu, Xiaokang1,2,6; Yang, Yuting6; Zhang, Fangyuan6; Sun, Xiaobo3; Zhang, Tong1; Guo, Dean2,5; Gong, Jiyu6; Yang, Zizhao1,4 |
| 刊名 | CHINESE MEDICINE
 |
| 出版日期 | 2025-12-18 |
| 卷号 | 20期号:1页码:25 |
| 关键词 | Panax Notoginseng Saponins (PNS)
Notoginsenoside R1 (NGR1)
|
| ISSN号 | 1749-8546 |
| DOI | 10.1186/s13020-025-01270-3 |
| 通讯作者 | Gong, Jiyu(gjy0431@126.com) ; Yang, Zizhao(zzyang@shutcm.edu.cn) |
| 英文摘要 | BackgroundIn recent years, skin sunburn injury caused by UVB has become a growing concern. Although PNS have demonstrated potential in alleviating this condition, the precise mechanisms involved remain incompletely elucidated.PurposeThis study was designed with three primary objectives. First, to apply network pharmacology-based predictive approaches to elucidate the mechanisms underlying PNS-mediated protection against UVB-induced skin sunburn injury. Second, to systematically analyze the chemical profile of PNS through UHPLC-Q-Orbitrap-MS/MS. Third, to conduct a comprehensive assessment of the pharmacodynamic properties of NGR1, a major bioactive constituent of PNS.MethodsThe chemical constituents of PNS were analyzed qualitatively and quantitatively using UHPLC and UHPLC-Q-Trap-MS/MS. Network pharmacology approaches were employed to identify the core molecular targets and potential mechanisms through which PNS alleviates UVB-induced sunburn injury. To evaluate the therapeutic effects of PNS and NGR1, an in vivo model was established using nude mice, while mechanistic studies were conducted in HaCaT cells to elucidate the underlying signaling pathways.ResultsA total of 16 primary saponins in PNS were successfully identified and quantified. Through network pharmacology analysis, 49 crucial molecular targets associated with PNS in the context of UVB-induced skin sunburn injury were revealed. Treatment with PNS and NGR1 ameliorated signs of photoaging via multiple mechanisms, including suppression of inflammatory responses, boosting antioxidant capacity, inhibition of the PI3K/AKT/mTOR signaling cascade, and regulation of proteins involved in maintaining cellular homeostasis. In HaCaT cells, PNS and NGR1 exert protective effects against apoptosis by modulating proteins associated with cellular homeostasis and autophagy. Both compounds counteracted the UVB-induced reduction in NAT10 expression. The degradation of NAT10, potentially mediated by the autophagy pathway involving key selective adaptors such as NBR1 and p62, may occur under both basal and UVB-exposed conditions.ConclusionPNS and NGR1 demonstrate promising therapeutic potential for the treatment of UVB-induced skin sunburn injury. Their capacity to mitigate photodamage via multiple mechanisms, such as inhibition of key signaling pathways, regulation of apoptosis and autophagy, and modulation of NAT10 expression, lays a strong foundation for future clinical studies on topical applications of PNS and NGR1, while also providing valuable insights into their preventive and curative effects.Graphical AbstractPNS prevents cell apoptosis and autophagy by suppressing the activation of the PI3K/AKT/mTOR signaling pathway, whereas NGR1 enhances the activity of RNA acetyltransferase NAT10 to exert its protective effects. Their combined action contributes to the recovery from UVB-induced skin sunburn injury. |
| WOS关键词 | INDUCED DNA-DAMAGE ; MATRIX METALLOPROTEINASE-1 ; GINSENOSIDE RC ; RADIATION ; IMPACT ; INFLAMMATION ; EXPRESSION ; CELLS ; MICE |
| 资助项目 | Shanghai Pujiang Program, China[23PJ1412300] ; Project of Taking on Challenges and Accepting Responsibilities of The Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine[QYCXZY250303] ; National Natural Science Foundation of China[82104494] |
| WOS研究方向 | Integrative & Complementary Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001641588200001 |
| 出版者 | BMC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322393]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Gong, Jiyu; Yang, Zizhao |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 3.Peking Union Med Coll & Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China 4.Shanghai Univ Tradit Chinese Med, Peoples Hosp 7, Ctr Lab Anim Serv & Expt, Shanghai 200137, Peoples R China 5.Shanghai Univ Tradit Chinese Med, Shanghai Int Standardizat Res Inst Tradit Chinese, Shanghai 201203, Peoples R China 6.Changchun Univ Chinese Med, Sch Pharmaceut Sci, Changchun 130117, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Shuyun,Liu, Xiaokang,Yang, Yuting,et al. Notoginsenoside R1 mitigates UVB-induced skin sunburn injury through modulation of N4-acetylcytidine and autophagy[J]. CHINESE MEDICINE,2025,20(1):25. |
| APA | Liang, Shuyun.,Liu, Xiaokang.,Yang, Yuting.,Zhang, Fangyuan.,Sun, Xiaobo.,...&Yang, Zizhao.(2025).Notoginsenoside R1 mitigates UVB-induced skin sunburn injury through modulation of N4-acetylcytidine and autophagy.CHINESE MEDICINE,20(1),25. |
| MLA | Liang, Shuyun,et al."Notoginsenoside R1 mitigates UVB-induced skin sunburn injury through modulation of N4-acetylcytidine and autophagy".CHINESE MEDICINE 20.1(2025):25. |
入库方式： OAI收割
来源：上海药物研究所
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