Integrated network pharmacology, metabolomics, and proteomics analysis of Shiqi Waigan Granules for treating exogenous febrile disease
文献类型：期刊论文
| 作者 | Feng, Lin2,3; Liu, Yongqi3; Liu, Xiaokang3; Fang, Ling3; Tang, Zijun3; Liu, Kangnan3; Li, Zhenwei3; Zhang, Daidi3; Lai, Minling1; Peng, Lihua4 |
| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
 |
| 出版日期 | 2026-03-01 |
| 卷号 | 358页码:17 |
| 关键词 | Shiqi Waigan Granules Network pharmacology Metabolomics Proteomics |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2025.121029 |
| 通讯作者 | Guo, De-an(daguo@shutcm.edu.cn) |
| 英文摘要 | Ethnopharmacological relevance: Fever, which is closely associated with inflammation, is classified in Traditional Chinese Medicine as either exogenous or endogenous. Shiqi Waigan Granules (SWG) are indicated for treating fever and headache caused by exogenous factors, as well as for preventing influenza. Aim of the study: To investigate the main therapeutic compounds and potential mechanisms of SWG in treating exogenous febrile disease. Materials and methods: UPLC-QTOF/MS profiling identified the absorbed compounds of SWG in rat plasma. Network pharmacology, metabolomics, and proteomics were employed to pinpoint the targets and pathways, while molecular docking, immunofluorescence, and Western blot validated these findings. Results: A total of 47 prototype compounds and 13 metabolites were detected in rat plasma. Network analysis identified 145 overlapping targets associated with antipyretic and anti-inflammatory GO and KEGG pathways. Molecular docking revealed that key bioactive compounds-Asprelloside B, Verbascoside, Isoverbascoside, Chlorogenic acid, and Ellagic acid 4-O-xylopyranoside-exhibited binding energies lower than -7.0 kcal mol- 1 with core proteins PIK3CA, AKT1, NF-kappa B, and I kappa B-alpha. SWG significantly reduced LPS-induced fever in rats, an effect likely mediated by suppression of pro-inflammatory cytokine production. Furthermore, SWG modulated pivotal metabolites identified through metabolomics, influencing the thermoregulatory center and exerting antipyretic effects. Key metabolic pathways involved included unsaturated fatty acid metabolism, lipid metabolism, and arachidonic acid metabolism. Proteomic analysis revealed differentially expressed proteins enriched in the NF-kappa B and PI3K/Akt signaling pathways. Western blot and immunofluorescence assays confirmed that SWG inhibited inflammatory activation by regulating the PI3K/Akt and NF-kappa B pathways. Conclusion: SWG exerts multi-compound, multi-target, multi-pathway control of exogenous febrile disease, offering a rational basis for its clinical application and novel strategies for fever management. |
| WOS关键词 | FEVER |
| 资助项目 | National Natural Science Foundation of China[82130111] ; Qi-Huang Chief Scientist Project of National Administration of Traditional Chinese Medicine |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001642426700001 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/322441]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, De-an |
| 作者单位 | 1.Zhongshan Hengsheng Pharmaceut Co Ltd, Zhongshan 528437, Peoples R China 2.Changchun Univ Chinese Med, Coll Pharm, Changchun 130117, Peoples R China 3.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 582400, Peoples R China 4.Zhongshan Zhongzhi Pharmaceut Grp Co Ltd, Zhongshan 528437, Peoples R China 5.Shanghai Univ Tradit Chinese Med, Shanghai Acad Int Standardizat Tradit Chinese Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Lin,Liu, Yongqi,Liu, Xiaokang,et al. Integrated network pharmacology, metabolomics, and proteomics analysis of Shiqi Waigan Granules for treating exogenous febrile disease[J]. JOURNAL OF ETHNOPHARMACOLOGY,2026,358:17. |
| APA | Feng, Lin.,Liu, Yongqi.,Liu, Xiaokang.,Fang, Ling.,Tang, Zijun.,...&Guo, De-an.(2026).Integrated network pharmacology, metabolomics, and proteomics analysis of Shiqi Waigan Granules for treating exogenous febrile disease.JOURNAL OF ETHNOPHARMACOLOGY,358,17. |
| MLA | Feng, Lin,et al."Integrated network pharmacology, metabolomics, and proteomics analysis of Shiqi Waigan Granules for treating exogenous febrile disease".JOURNAL OF ETHNOPHARMACOLOGY 358(2026):17. |
入库方式： OAI收割
来源：上海药物研究所
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