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Integrated metabolome and transcriptome analyses reveal the seasonal dynamic accumulation and regulation network of benzylisoquinoline alkaloids in Stephania tetrandra roots

文献类型：期刊论文

作者Hu, Jiqiang2,3; Tu, Nannan3; Lin, Cuiying3; Fan, Yuzhe3; Xu, Zhijian; Yao, Jianbiao1; Zeng, Su3; Yang, Bingxian4,5; Sun, Lianli3
刊名FOOD BIOSCIENCE
出版日期2026
卷号75页码:14
关键词Benzylisoquinoline alkaloids Stephania tetrandra Metabolome Transcriptome Abscisic acid Reactive oxygen species
ISSN号2212-4292
DOI10.1016/j.fbio.2025.108177
英文摘要Stephania tetrandra is a medicinal plant that contains four main types of benzylisoquinoline alkaloids (BIAs): mono-BIAs, bis-BIAs, aporphine-type BIAs (APT-BIA), and tetrahydroprotoberberines (THBs). S. tetrandra is particularly rich in the bis-BIAs, tetrandrine and fangchinoline, which are critical quality markers and major components of the traditional Chinese medicine "Fangji". However, changes in their BIA profiles and associated metabolic regulatory networks over time remain unclear. In this study, metabolomics and transcriptomics were integrated to investigate the dynamic accumulation patterns and underlying mechanisms of these alkaloids across three developmental stages: Autumnal Equinox (AuEq), Winter Solstice (WinSo), and Vernal Equinox (VeEq). Quantification revealed that both alkaloids reached their highest concentrations at VeEq, with tetrandrine levels more than doubling from WinSo. Untargeted metabolomics identified 61 differentially accumulated BIAs, displaying stage-specific accumulation patterns. Hierarchical clustering grouped these BIAs into four structural categories, predominantly enriched at VeEq. Transcriptome profiling showed significant upregulation of genes involved in isoquinoline alkaloid biosynthesis during VeEq, especially key enzymes such as TyrAT, TYDC, 4HPPDC, and NCS. Co-expression and protein interaction network analyses further identified abscisic acid (ABA) signaling pathways as major hormonal regulators. Notably, ABA levels and exogenous ABA application confirmed its pivotal role in promoting bis-BIA biosynthesis, acting in concert with reactive oxygen species (ROS) to fine-tune alkaloid production. Collectively, this study establishes a hormone-ROS-BIA regulatory framework that explains seasonal fluctuations in alkaloid content and provides new entry points for improving the medicinal quality and cultivation strategies of S. tetrandra.
WOS关键词BIOSYNTHESIS ; QUANTIFICATION ; ARABIDOPSIS ; GLUCOSE ; RNA
资助项目Zhejiang Provincial Natural Science Foundation of China[LY24B020001] ; Zhejiang Province Science and Technology Program[2022C03142]
WOS研究方向Food Science & Technology
语种英语
WOS记录号WOS:001655992500001
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/322566]  
专题国家级研究中心_原创新药研究全国重点实验室
通讯作者Yang, Bingxian; Sun, Lianli
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Drug Discovery & Design Ctr, Shanghai 201203, Peoples R China
2.Conba Grp Co Ltd, Hangzhou 310052, Peoples R China
3.Zhejiang Univ, Inst Drug Metab & Pharmaceut Anal, Coll Pharmaceut Sci, Hangzhou 310058, Peoples R China
4.Zhejiang Sci Tech Univ, Coll Life Sci & Med, Hangzhou 310018, Peoples R China
5.Zhejiang CONBA Pharmaceut Co Ltd, Hangzhou 310052, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Jiqiang,Tu, Nannan,Lin, Cuiying,et al. Integrated metabolome and transcriptome analyses reveal the seasonal dynamic accumulation and regulation network of benzylisoquinoline alkaloids in Stephania tetrandra roots[J]. FOOD BIOSCIENCE,2026,75:14.
APA Hu, Jiqiang.,Tu, Nannan.,Lin, Cuiying.,Fan, Yuzhe.,Xu, Zhijian.,...&Sun, Lianli.(2026).Integrated metabolome and transcriptome analyses reveal the seasonal dynamic accumulation and regulation network of benzylisoquinoline alkaloids in Stephania tetrandra roots.FOOD BIOSCIENCE,75,14.
MLA Hu, Jiqiang,et al."Integrated metabolome and transcriptome analyses reveal the seasonal dynamic accumulation and regulation network of benzylisoquinoline alkaloids in Stephania tetrandra roots".FOOD BIOSCIENCE 75(2026):14.

入库方式： OAI收割

来源：上海药物研究所

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